Abstract

We are examining both temporal and spatial changes in protein tyrosine phosphorylation following fertilization of mouse eggs using an antibody that recognizes phosphotyrosine and a secondary rhodamine or fluorescein conjugated antibody. In addition, we are using primary antibodies that recognize specific acetylated isoforms of histone-H4 to determine temporal and spatial changes in these proteins during preimplantation development in the mouse. 8-31-96

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002483-10
Application #
5224059
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Deng, Manqi; Williams, Carmen J; Schultz, Richard M (2005) Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity. Dev Biol 278:358-66
Tutuncu, Levent; Stein, Paula; Ord, Teri S et al. (2004) Calreticulin on the mouse egg surface mediates transmembrane signaling linked to cell cycle resumption. Dev Biol 270:246-60
Brown, Rebecca L; August, Shelley L; Williams, Carmen J et al. (2003) AKAP7gamma is a nuclear RI-binding AKAP. Biochem Biophys Res Commun 306:394-401
Deng, Manqi; Kishikawa, Hidefumi; Yanagimachi, Ryuzo et al. (2003) Chromatin-mediated cortical granule redistribution is responsible for the formation of the cortical granule-free domain in mouse eggs. Dev Biol 257:166-76
Brown, Rebecca L; Ord, Teri; Moss, Stuart B et al. (2002) A-kinase anchor proteins as potential regulators of protein kinase A function in oocytes. Biol Reprod 67:981-7
Li, J; Zhu, X; Ashton, F T et al. (2001) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the third-stage larva. J Parasitol 87:65-72
Robson, P; Stein, P; Zhou, B et al. (2001) Inner cell mass-specific expression of a cell adhesion molecule (PECAM-1/CD31) in the mouse blastocyst. Dev Biol 234:317-29
Travis, A J; Merdiushev, T; Vargas, L A et al. (2001) Expression and localization of caveolin-1, and the presence of membrane rafts, in mouse and Guinea pig spermatozoa. Dev Biol 240:599-610
Stein, P; Schultz, R M (2000) Initiation of a chromatin-based transcriptionally repressive state in the preimplantation mouse embryo: lack of a primary role for expression of somatic histone H1. Mol Reprod Dev 55:241-8
Li, J; Ashton, F T; Gamble, H R et al. (2000) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the first stage larva. J Comp Neurol 417:299-314

Showing the most recent 10 out of 38 publications