Abstract

Centractin is an actin-related protein that shares 53% homology with conventional actin and has a similar nucleotide binding core. Biochemical studies show that centractin in a member of a stable, polypeptide complex named dynactin, which itself has been implicated in activating dynein mediated vesicle transport along microtubules. We are using transient transfection assays to investigate the effects of overexpressing centractin in PtK2 cells, studying the result by confocal fluorescent immunomicroscopy using antibodies to centractin and other cytoskeletal proteins plus fluorescent phalloidin for filamentous actin. Two distinct patterns of centractin localization are consistently observed; filamentous centractin located throughout the cytoplasm, and perinuclear accumulations. The latter are curved and highly three-dimensional. To further explore the nature of the perinuclear accumulations, we applied our methods for sequential confocal fluorescence and IVEM of the same cell preparations. The result is that the perinuclear fluorescence co-localizes with an apparently membranous structure near the nucleus that has the morphology of the Golgi apparatus. 8-31-96

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002483-10
Application #
5224065
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Deng, Manqi; Williams, Carmen J; Schultz, Richard M (2005) Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity. Dev Biol 278:358-66
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Deng, Manqi; Kishikawa, Hidefumi; Yanagimachi, Ryuzo et al. (2003) Chromatin-mediated cortical granule redistribution is responsible for the formation of the cortical granule-free domain in mouse eggs. Dev Biol 257:166-76
Brown, Rebecca L; Ord, Teri; Moss, Stuart B et al. (2002) A-kinase anchor proteins as potential regulators of protein kinase A function in oocytes. Biol Reprod 67:981-7
Li, J; Zhu, X; Ashton, F T et al. (2001) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the third-stage larva. J Parasitol 87:65-72
Robson, P; Stein, P; Zhou, B et al. (2001) Inner cell mass-specific expression of a cell adhesion molecule (PECAM-1/CD31) in the mouse blastocyst. Dev Biol 234:317-29
Travis, A J; Merdiushev, T; Vargas, L A et al. (2001) Expression and localization of caveolin-1, and the presence of membrane rafts, in mouse and Guinea pig spermatozoa. Dev Biol 240:599-610
Stein, P; Schultz, R M (2000) Initiation of a chromatin-based transcriptionally repressive state in the preimplantation mouse embryo: lack of a primary role for expression of somatic histone H1. Mol Reprod Dev 55:241-8
Li, J; Ashton, F T; Gamble, H R et al. (2000) Sensory neuroanatomy of a passively ingested nematode parasite, Haemonchus contortus: amphidial neurons of the first stage larva. J Comp Neurol 417:299-314

Showing the most recent 10 out of 38 publications