This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Our long-term goal to translate much of our early 13C isotopomer methods to in vivo metabolic studies for clinical applications is now a realistic possibility. Thus, the focus of the renewed support will be to investigate fundamental metabolic questions using hyperpolarized 13 C MR measurements in isolated, perfused rat hearts and mouse livers with the intent to translate these findings into direct clinical practice. As in the past, we believe these fundamental studies are extremely important as we begin to apply these latest MR technologies to the study of human metabolic diseases. This project has four specific aims: 1) Develop hyperpolarized 13C methods to measure flux through individual enzyme catalyzed steps and through specific spans of the TCA cycle. 2) Develop a hyperpolarized 13C method to measure gluconeogenesis in isolated perfused mouse livers. 3) Develop a hyperpolarized 13C method to measure flux through the pentose phosphate pathway in perfused hearts and livers. 4) Develop the tools necessary to image tissue red-ox by 13C MRS using hyperpolarized redox indicators.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002584-21
Application #
7724116
Study Section
Special Emphasis Panel (ZRG1-SBIB-Q (40))
Project Start
2008-09-01
Project End
2009-08-31
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
21
Fiscal Year
2008
Total Cost
$10,458
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
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