Existence of left handed DNA (Z-DNA) was discovered and extensively characterized in 1979. Since then there has been considerable research in establishing biological roles for Z-DNA. Recently Rich's group has established that a human protein, ds RNA deminase (dsRAD) has specificity for binding Z-DNA. The goal of this project is to provide direct evidence for the existence of left handed conformation for DNA when it binds to protein ds RAD. Raman spectra of poly(GC) in low salt and high salt solution, control protein, protein/poly (GC) complex have been obtained using 407 nm laser excitation. Raman spectral features of left and right handed DNA conformers have been characterized. By comparing these with the spectra of the protein/ poly (GC) complex, we concluded that DNA exists in left handed conformer when it binds to the ds RAD protein. Additional evidence have been provided by fluorescence and CD spectroscopy.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002594-14
Application #
6121257
Study Section
Project Start
1999-06-01
Project End
2000-05-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
14
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
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