This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Epidermis displays two unique and characteristic electrolytic gradients: First, a calcium (Ca2+) gradient, which regulates numerous epidermal functions via cell-physiologic processes and effects on differentiation. Second, the acidic pH of the SC also forms a functional endpoint late in epidermal differentiation, which regulates epidermal functions such as extracellular lipid processing and desquamation. Using Fluorescence Lifetime Imaging Microscopy (FLIM), both pH and Ca2+ concentration gradients and subcellular localization within the epidermis will be investigated concurrently, with the ultimate goal not only to determine how these two gradients are linked, but also how they are regulated by epidermal tight junctions and how they may be altered in certain disease states.
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