The overall goal of this project is to understand the cellular mechanisms used by neuromodulators to control behavioural plasticity. Specifically, we are using multiphoton microscopy and electrophysiology to study the cellular mechanisms by which neuromodulators produce various motor patterns in a small, well characterized, neural network-- the lobster stomatogastric ganglion. Neuromodulators such as dopamine and serotonin play central roles in the regulation of behavior in both vertebrates and invertebrates. The Harris-Warrick group has analyzed the effects of dopamine, serotonin and octopamine on the well-studied 14-cell pyloric network in the crustacean stomatogastric ganglion (STG). These compounds are endogenous neuromodulators of the pyloric network and when bath-applied to the stomatogastric ganglion, each amine evokes a unique variant on the rhythmic motor pattern generated by the pyloric network, with changes in the cycle frequency, active neurons, phasing and intensity of n euronal firing. We are currently using multiphoton microscopy measure of calcium signals and monitor the time course and spatial distribution of the calcium changes in STG neurons elicited by amines. These experiments are designed to answer two major questions: (1) do amines modify calcium entry and/or release from intracellular stores, and (2) are the actions of amines on calcium activity different at nerve terminals (where they might affect transmitter release) than in other regions of the cell where calcium might modulate the intrinsic firing properties of the cell?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR004224-15
Application #
6657765
Study Section
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
15
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Cornell University
Department
Type
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Migone, Fernando F; Cowan, Robert G; Williams, Rebecca M et al. (2016) In vivo imaging reveals an essential role of vasoconstriction in rupture of the ovarian follicle at ovulation. Proc Natl Acad Sci U S A 113:2294-9
O'Dell, Ryan S; Cameron, David A; Zipfel, Warren R et al. (2015) Reelin Prevents Apical Neurite Retraction during Terminal Translocation and Dendrite Initiation. J Neurosci 35:10659-74
Byrnes, Laura J; Singh, Avtar; Szeto, Kylan et al. (2013) Structural basis for conformational switching and GTP loading of the large G protein atlastin. EMBO J 32:369-84
Jain, Manu; Robinson, Brian D; Scherr, Douglas S et al. (2012) Multiphoton microscopy in the evaluation of human bladder biopsies. Arch Pathol Lab Med 136:517-26
Degala, Satish; Williams, Rebecca; Zipfel, Warren et al. (2012) Calcium signaling in response to fluid flow by chondrocytes in 3D alginate culture. J Orthop Res 30:793-9
O'Dell, Ryan S; Ustine, Candida J M; Cameron, David A et al. (2012) Layer 6 cortical neurons require Reelin-Dab1 signaling for cellular orientation, Golgi deployment, and directed neurite growth into the marginal zone. Neural Dev 7:25
McMullen, J D; Kwan, A C; Williams, R M et al. (2011) Enhancing collection efficiency in large field of view multiphoton microscopy. J Microsc 241:119-24
Kim, Sally A; Sanabria, Hugo; Digman, Michelle A et al. (2010) Quantifying translational mobility in neurons: comparison between current optical techniques. J Neurosci 30:16409-16
Bowles, Robby D; Williams, Rebecca M; Zipfel, Warren R et al. (2010) Self-assembly of aligned tissue-engineered annulus fibrosus and intervertebral disc composite via collagen gel contraction. Tissue Eng Part A 16:1339-48
McMullen, Jesse D; Zipfel, Warren R (2010) A multiphoton objective design with incorporated beam splitter for enhanced fluorescence collection. Opt Express 18:5390-8

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