This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a pilot study to assess the appropriateness of using MRI to localize electrode tracts in the brain of nonhuman primates.
We aim to collect high-resolution structural images of the tracts left by micro-electrode arrays (with individual microwires of 50 microns in diameter with 300 micron spacing) in multiple cortical regions in the brain of a macaque. Our goal is to effectively localize the electrode tracts in 3-D with respect to anatomical landmarks. Traditional histological procedures, while effective, can distort tissue making electrode tract reconstruction difficult. Because the tissue is cut, utility of the tissue specimin for additional analysis is diminished. Imaging is important as the original specimin can be studied without damage and remain available for further study. The specimen is the preserved brain of a female rhesus macaque (macaca mulatta) which was chronically implanted (>18 months) with microwire electrode arrays in multiple cortical areas. The brain will be extracted from the skull and scanned in two ways: first, in its native formalin-preserved state and subsequently after soaking in buffered saline and ProHance for 7-10 days. T1 and T2 weighted MRI images will be acquired.
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