This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.To ultrasound image the hearts of mice exposed to hypobaric (altitude up to 24000ft) conditions for up to 14 days in duration. To start, we would like to do 12 mice at 0, 7, and 14 days. The purpose of our project is to measure the effect of environmental stressors on cardiac function in the mouse. Our stresses include one hour of 500ppm carbon monoxide (CO) and hypoxia, initially in wild type mice, and eventually in eNOS gene knockout mice. Our hypothesis is that the two physiological gases play complementary roles in cardiac mitochondrial biogenesis that are different from hypoxia and have implication for the functioning mouse heart. We are currently performing cardiac ultrasound studies consist of the following: -- Each mouse heart is imaged at the following time points: Control (day 0 pre-exposure. e.g. CO), 7 days post CO, 14 days post CO -- Both left and right ventricles are scanned in the long and short axis at low and high resolution Measurements are then made of each high resolution scan at diastole and systole to determine the area of the ventricular chamber. -- The areas are then used to determine the ejection fraction: EF=(AD-AS)/AD)
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