Ion fluxes and ATPase activity are studied in intact cells,resealed ghosts and cell-free membranes, and 31P NMR spectroscopyis used to follow energetics in whole cells. Effects oftemperature on these measurements are studied in red cells of coldsensitive (human) and insensitive (ground squirrel) species. Wehave demonstrated that species differences in the effects oftemperature on free intracellular magnesium can account for muchif not all of the difference in temperature sensitivity of theNa/K pump. Studies using 19F NMR and a fluorinated calciumindicator have now begun in order to measure changes inintracellular free calcium as a function of

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR005964-06A1
Application #
5225181
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost
Li, Li; Palencia, Andrés; Lukk, Tiit et al. (2013) Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens. Proc Natl Acad Sci U S A 110:3817-22
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Sallam, Khalid Ibrahim; Samejima, Kunihiko (2004) Effects of Trisodium Phosphate and Sodium Chloride Dipping on the Microbial Quality and Shelf Life of Refrigerated Tray-packaged Chicken Breasts. Food Sci Biotechnol 13:425-429
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