This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Keywords to describe research content: RPSC, RPS12 Keywords to describe computational methods used: Clustar, MSA Abstract: This semester students in my BIOL302L (Molecular and General Genetics Lab) have selected for Streptomycin mutants in E.coli. The mutations were scored to see if the mutation resulted in resistance to streptomycin or dependence on it. Rpsl encodes the 30s ribosomal protein, rps12. The mutations which survive in streptomycin must be able to translate mRNA accurately, so the mutation has to be change the protein enough so that either streptomycin does not bind or so that binding to streptomycin allows translation. Thus we expect the mutants to differ slightly from the wild type protein and that the amino acid changes will be very restricted. The students sequenced rpsl and now have a bank of sequences. They will compare sequences of the mutants to the published wildtype sequence. The intention is that they model the mutants and visualize the differences in the proteins.
Showing the most recent 10 out of 292 publications