This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The isolation of rare cells (those present at low frequency) is important in diagnostic medicine. The use of antibody coated surfaces to capture specific cell types has been thoroughly studied but collision frequencies and high probabilities of detachment before capture is complete are more important when the target cells are rare. For surface capture to be effective, cells need to make physicochemical contact with the antibody coated entities. Because cells suspended in medium cannot be considered as point masses, the usual computational fluid dynamics (CFD) techniques of tracking particle movement are insufficient to predict the frequency of contact. The novel Macroscopic Particle Model recently proposed by Fluent developers will be used and extended to relate its parameters to collision frequencies and patterns known to occur in experimentally observed systems. The results will be used to estimate and analyze rare capture events which are considerably harder to measure.
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