This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a continuation of GUP-2492, which is a collaborative project with Dr. Keith Moffett and Dr. Spencer Andersen to undertake crystallographic analysis of a newly identified photoreceptor class called the BluF family of photreceptors. . Previous MAD data collected on beam 14-BM-D and subsequent higher resolution data set on 14-BM-C has lead to a high resolution structure of the dark form of AppA whiich was published in Biochemistrty. A second study has yielded a high resolution structure of a related photoreceptor called Slr1694 from Synechocystis. This second structure of this is ready for submission for publication (a manuscript has been written and just needs approval for submission by Drs. Anderson and Moffat who are coauthors). Recently we have generated a number of crystals (0.3 to 0.7 mm) of AppA that undergo transition from a dark ground state to a light excited state upon illumination Higher resolution data that can be provided on14-BM-C, is requested to determine what structural rearrangements are occurring between dark and light adapted forms of this photoreceptor. The goal of this proposal wil be to obtain the light excited structure of AppA that will be compaired to the dark structure to determine what type of conformational change occures in this photoreceptor upon illumination. Both AppA and Slr1694 represent a new novel class of photoreceptor that will require the collection of high resolution data collected from both dark and light excited crystals in order to reveal the molecular mechanism light mediated changes in protein structure. AppA and Slr1649 represents a newly discovered class of novel photoreceptors that have been observed in over 20 sequenced bacterial genomes (including many non-photosynthetic organisms such as E. coli) as well as in eukaryotic algal genomes. The photocycle of this class of photoreceptors is very long-lived ranging from 1.5 seconds to 30 min in duration. This is very different from other photorecept
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| Yang, Cheolhee; Choi, Minseo; Kim, Jong Goo et al. (2018) Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering. Int J Mol Sci 19: |
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| Pande, Kanupriya; Hutchison, Christopher D M; Groenhof, Gerrit et al. (2016) Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein. Science 352:725-9 |
| Weingarten, Adam S; Kazantsev, Roman V; Palmer, Liam C et al. (2015) Supramolecular Packing Controls H? Photocatalysis in Chromophore Amphiphile Hydrogels. J Am Chem Soc 137:15241-6 |
| Pfoh, Roland; Pai, Emil F; Saridakis, Vivian (2015) Nicotinamide mononucleotide adenylyltransferase displays alternate binding modes for nicotinamide nucleotides. Acta Crystallogr D Biol Crystallogr 71:2032-9 |
| Mariette, Céline; Guérin, Laurent; Rabiller, Philippe et al. (2015) The creation of modulated monoclinic aperiodic composites in n-alkane/urea compounds. Z Kristallogr Cryst Mater 230:5-11 |
| Yang, Xiaojing; Stojkovi?, Emina A; Ozarowski, Wesley B et al. (2015) Light Signaling Mechanism of Two Tandem Bacteriophytochromes. Structure 23:1179-89 |
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