Light quenching is a unique opportunity to increase the resolution in fluorescence light microscopy. The resolution of a fluorescence light microscopy can be increased up to about 35 fm by quenching the excited molecules at the outer regions of the focused light spot, as has been shown in a theoretical study. For the practical development of such a microscopy the light quenching properties and their study are of essential interest. The goal of the present studies is to define and measure the amount of light quenching depending on the wavelength and intensity of the beams used. It will be of utmost interest to find dyes working in the 1R regime since these dyes can easily be exploited in combination with the easy-to-use Titanium-Sapphire lasers. It is also interesting to study the lifetimes of the dyes in order to optimize the setup for a maximum of light quenching.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-04
Application #
5225701
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost
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