Abstract

Understanding the mechanism of hemoglobin dissociation and association is a fundamental factor to study hemoglobin stability and production of hemoglobin based extracellular oxygen carriers. Clinical studies with different crosslinked and modified hemoglobins reveled symptoms which are due to fast kidney filtration and accelerated autoxidation. We are utilizing ultrafast time-resolved intrinsic tryptophan fluorescence for monitoring the hemoglobin dissociation into the dimers and monomers under high hydrostatic pressure. Our study include native human hemoglobin as well as crosslinked and mutated human and bovine hemoglobins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR008119-06
Application #
6282887
Study Section
Project Start
1998-04-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
Hartman, Kira G; McKnight, Laura E; Liriano, Melissa A et al. (2013) The evolution of S100B inhibitors for the treatment of malignant melanoma. Future Med Chem 5:97-109
Fu, Yi; Zhang, Jian; Lakowicz, Joseph R (2009) Highly efficient detection of single fluorophores in blood serum samples with high autofluorescence. Photochem Photobiol 85:646-51
Zhang, Jian; Badugu, Ramachandram; Lakowicz, Joseph R (2008) Fluorescence Quenching of CdTe Nanocrystals by Bound Gold Nanoparticles in Aqueous Solution. Plasmonics 3:3-11
Ray, Krishanu; Badugu, Ramachandram; Lakowicz, Joseph R (2007) Polyelectrolyte Layer-by-Layer Assembly To Control the Distance between Fluorophores and Plasmonic Nanostructures. Chem Mater 19:5902-5909
Matveeva, Evgenia G; Gryczynski, Ignacy; Barnett, Anne et al. (2007) Metal particle-enhanced fluorescent immunoassays on metal mirrors. Anal Biochem 363:239-45
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles. Nano Lett 7:2101-7
Zhang, Jian; Fu, Yi; Chowdhury, Mustafa H et al. (2007) Single-Molecule Studies on Fluorescently Labeled Silver Particles: Effects of Particle Size. J Phys Chem C Nanomater Interfaces 112:18
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Single cell fluorescence imaging using metal plasmon-coupled probe. Bioconjug Chem 18:800-5
Zhang, Jian; Fu, Yi; Lakowicz, Joseph R (2007) Emission Behavior of Fluorescently Labeled Silver Nanoshell: Enhanced Self-Quenching by Metal Nanostructure. J Phys Chem C Nanomater Interfaces 111:1955-1961

Showing the most recent 10 out of 44 publications