We examined the intensity and anisotropy decay of DNA labeled with two ruthenium metal-ligand complexes, [Ru(bpy)2(dppz)]2+ and [Ru(phe)2(dppz)]2+. Both complexes display high emission anisotropies in the asence of rotational diffusion, making them suitable probes for rotational motions. When bound to DNA, this complex display decay time as long as 294 ns, providing long living probes for DNA. The use of metal-ligand complexes allows resolution of both the torsional and bending motions of DNA.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR008119-06
Application #
6282908
Study Section
Project Start
1998-04-01
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
6
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
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