Green fluorescent proteins has become widely used to study gene expression, in cells and even in whole mammalian organisms. Two-Photon induced fluorescence of Green Fluorescence Protein (GFP) mutant was observed and characterized. Unexpectedly we have found that two-photon cross-section of this GFP mutant is enormously high, several time higher than for wild type GFP. The lifetime and anisotropy decays were measured with one and two photon excitation and analyzed globally. The zero time anisotropy for two photon excitation is large than for one photon excitation by the factor 10/7.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR008119-09
Application #
6444714
Study Section
Project Start
2001-04-01
Project End
2002-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
9
Fiscal Year
2001
Total Cost
$293,069
Indirect Cost
Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Piao, Wenji; Shirey, Kari Ann; Ru, Lisa W et al. (2015) A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza. Cell Rep 11:1941-52
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