Introduction: Diffusion- (DWI) and perfusion-weighted (PWI) magnetic resonance imaging are powerful tools for the evaluation of therapeutic drugs for stroke. The temporal progression of the ischemic lesion can be monitored by DWI whereas PWI can be used to follow the underlying hemodynamics. The purpose of this study was to evaluate the efficacy of a novel intra-vascular compound, polynitroxyl albumin (PNA) (SynZyme Technologies), in a transient focal cerebral ischemia model in the rat. Methods: Anesthetized Sprague-Dawley rats underwent 2 hours of middle cerebral artery occlusion (MCAO) by an intraluminal suture. Three treatment groups (N=6 per group) were studied: i) saline and ii) albumin as controls, and iii) PNA. PNA, albumin, or saline was injected i.v. at 0.5, 2, and 4 h post-MCAO (total dose = 1% body weight). DWI and PWI (bolus tracking) were performed on a GE CSI 2 T spectrometer at 0.25 (before first drug treatment), 1, 1.7, 2.3, 3, and 4 h post-MCAO. At 24 h, brain sections were stained with 2,3,5-triphenyltetrazolium chloride (TTC) to obtain an MRI-independent measure of ischemic injury. Results and Conclusion: The ability of DWI to monitor the ischemic region over time revealed different evolution patterns. Lesion volumes increased significantly for both the saline- and albumin groups, whereas the growth of the lesion was suppressed in the PNA group. The lesion volume (4 h DWI) in the PNA group was reduced by 63% compared to the saline group (p=0.003) and by 52% compared to the albumin group (p = 0.006). The TTC-derived lesion volumes at 24 h are in reasonable agreement with the DWI at 4 h. The perfusion measurements suggest that improved blood flow pre- and post-reperfusion may be responsible for the beneficial effect of PNA.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR009784-05
Application #
6123024
Study Section
Project Start
1999-01-01
Project End
2000-07-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
Maclaren, Julian; Aksoy, Murat; Ooi, Melvyn B et al. (2018) Prospective motion correction using coil-mounted cameras: Cross-calibration considerations. Magn Reson Med 79:1911-1921
Guo, Jia; Holdsworth, Samantha J; Fan, Audrey P et al. (2018) Comparing accuracy and reproducibility of sequential and Hadamard-encoded multidelay pseudocontinuous arterial spin labeling for measuring cerebral blood flow and arterial transit time in healthy subjects: A simulation and in vivo study. J Magn Reson Imaging 47:1119-1132
Tamir, Jonathan I; Uecker, Martin; Chen, Weitian et al. (2017) T2 shuffling: Sharp, multicontrast, volumetric fast spin-echo imaging. Magn Reson Med 77:180-195
Lai, Lillian M; Cheng, Joseph Y; Alley, Marcus T et al. (2017) Feasibility of ferumoxytol-enhanced neonatal and young infant cardiac MRI without general anesthesia. J Magn Reson Imaging 45:1407-1418
Taviani, Valentina; Alley, Marcus T; Banerjee, Suchandrima et al. (2017) High-resolution diffusion-weighted imaging of the breast with multiband 2D radiofrequency pulses and a generalized parallel imaging reconstruction. Magn Reson Med 77:209-220
Uecker, Martin; Lustig, Michael (2017) Estimating absolute-phase maps using ESPIRiT and virtual conjugate coils. Magn Reson Med 77:1201-1207
Kogan, Feliks; Hargreaves, Brian A; Gold, Garry E (2017) Volumetric multislice gagCEST imaging of articular cartilage: Optimization and comparison with T1rho. Magn Reson Med 77:1134-1141
Aksoy, Murat; Maclaren, Julian; Bammer, Roland (2017) Prospective motion correction for 3D pseudo-continuous arterial spin labeling using an external optical tracking system. Magn Reson Imaging 39:44-52
Bian, W; Tranvinh, E; Tourdias, T et al. (2016) In Vivo 7T MR Quantitative Susceptibility Mapping Reveals Opposite Susceptibility Contrast between Cortical and White Matter Lesions in Multiple Sclerosis. AJNR Am J Neuroradiol 37:1808-1815
Vos, Sjoerd B; Aksoy, Murat; Han, Zhaoying et al. (2016) Trade-off between angular and spatial resolutions in in vivo fiber tractography. Neuroimage 129:117-132

Showing the most recent 10 out of 446 publications