This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A triple quadrupole electrospray ionization source, provided by MDS Sciex, has been integrated with a home built Fourier transform mass spectrometer. This system allows selected accumulation of low abundance post-translationally modified peptides, Q2-CAD of selected ions, top-down protein sequencing, and ECD/SORI-CAD/IRMPD in the ICR cell. These multiple fragmentation methods are used to obtain peptide sequence information with high resolution FTMS data. Sub-femtomole sensitivity and high transfer efficiency are possible. Further upgrades to the instrument have been designed and are ready for installation. These upgrades include the installation of a rail system for the support and alignment of all ion-optics and the ICR cell and the installation of a new cell that will increase the range of masses that can be trapped in the cell during each experiment as well as increase the capacity of the cell. The placement and mounting of the ECD gun will also be modified to reduce the impact of heat generated by the gun on the ICR cell. Upgrades have been added to the instrument including a heated metal capillary and oil mist filters - which have improved performance and stability of the instrument. The instrument now has 95% uptime and is generating data for a number of collaborative projects.
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