Abstract

General anesthesia is known to alter cardiopulmonary and metabolic parameters which affect tissue oxygenation. We have examined the changes of cerebral tissue pO2 and physiological parameters after injection of anesthetics in rats using EPR oximetry. Two dosages of ketamine/xylazine (100/10mg/kg and 50/5mg/kg i.m.) were investigated as a first stage for this study. Animals were allowed to breathe spontaneously. Cerebral tissue pO2 in both groups was almost constant until awakening ( 13.6, 11.9, 12.1, 12.4, 13.3, 13.6 mmHg at 30, 60, 90, 120,150, 180 min after injection respectively in high dosage group; 16.5, 15.4, 16.4 mmHg at 30, 60, 90 min in low dosage group). These pO2 values were extremely low compared with the value in an unanesthetized state (30~35mmHg) which we previously measured. The change of mean arterial pressure corresponded with that of cerebral pO2 . These data indicate that ketamine/xylazine anesthesia causes a substantial decrease of cerebral pO2 and its effect still remains at the awakening period. The experimental approach was extended to include the use of ventilation and various potential pressures of gas. Data then were obtained in foru different anesthetics: isoflurane, halothan, pentobarbital and ketamine/xylazine. See Highlight #6 for details.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR011602-03
Application #
6123390
Study Section
Project Start
1998-09-15
Project End
1999-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755

  Publications

Sinha, Birandra K; Leinisch, Fabian; Bhattacharjee, Suchandra et al. (2014) DNA cleavage and detection of DNA radicals formed from hydralazine and copper (II) by ESR and immuno-spin trapping. Chem Res Toxicol 27:674-82
Dunn, Jeff F; Khan, Mohammad N; Hou, Huagang G et al. (2011) Cerebral oxygenation in awake rats during acclimation and deacclimation to hypoxia: an in vivo electron paramagnetic resonance study. High Alt Med Biol 12:71-7
Shen, Jiangang; Khan, Nadeem; Lewis, Lionel D et al. (2003) Oxygen consumption rates and oxygen concentration in molt-4 cells and their mtDNA depleted (rho0) mutants. Biophys J 84:1291-8
Khan, Nadeem; Wilmot, Carmen M; Rosen, Gerald M et al. (2003) Spin traps: in vitro toxicity and stability of radical adducts. Free Radic Biol Med 34:1473-81
Pogue, Brian W; O'Hara, Julia A; Demidenko, Eugene et al. (2003) Photodynamic therapy with verteporfin in the radiation-induced fibrosarcoma-1 tumor causes enhanced radiation sensitivity. Cancer Res 63:1025-33
Hou, Huagang; Grinberg, Oleg Y; Taie, Satoshi et al. (2003) Electron paramagnetic resonance assessment of brain tissue oxygen tension in anesthetized rats. Anesth Analg 96:1467-72, table of contents
Chen, Bin; Pogue, Brian W; Goodwin, Isak A et al. (2003) Blood flow dynamics after photodynamic therapy with verteporfin in the RIF-1 tumor. Radiat Res 160:452-9
Dunn, Jeff F; Swartz, Harold M (2003) In vivo electron paramagnetic resonance oximetry with particulate materials. Methods 30:159-66
Khan, Nadeem; Shen, Jiangang; Chang, Ta Yuan et al. (2003) Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism. Biochemistry 42:23-9
Swartz, Harold M (2002) Measuring real levels of oxygen in vivo: opportunities and challenges. Biochem Soc Trans 30:248-52

Showing the most recent 10 out of 44 publications