Abstract

There is accumulating evidence for delayed genomic instability following radiation exposure.
Specific aims are: (1) to investigate directly whether the origination event is located intra- or extra-nuclearly to study delayed chromosomal instability in a hamster -human hybrid cell line using fluorescence in situ hybridization to examine populations of metaphase cells several generations after expanding single-cell colonies that had survived five or ten alpha particles either to the nucleus or to the cytoplasm. Delayed chromosomal instability, manifested as multiple rearrangements of human chromosome 4 in a background of hamster chromosomes, will be observed. For both irradiated groups, correlations will be investigated between delayed chromosomal instability and delayed reproductive cell death, manifested as reduced plating efficiency in surviving clones: such correlations would suggest a role for chromosome rearrangements in cytotoxicity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
1P41RR011623-01A1
Application #
5226004
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Chai, Y; Calaf, G M; Zhou, H et al. (2013) Radiation induced COX-2 expression and mutagenesis at non-targeted lung tissues of gpt delta transgenic mice. Br J Cancer 108:91-8
Chai, Y; Lam, R K K; Calaf, G M et al. (2013) Radiation-induced non-targeted response in vivo: role of the TGFýý-TGFBR1-COX-2 signalling pathway. Br J Cancer 108:1106-12
Hu, Burong; Grabham, Peter; Nie, Jing et al. (2012) Intrachromosomal changes and genomic instability in site-specific microbeam-irradiated and bystander human-hamster hybrid cells. Radiat Res 177:25-34
Hei, Tom K; Zhao, Yongliang; Zhou, Hongning et al. (2011) Mechanism of radiation carcinogenesis: role of the TGFBI gene and the inflammatory signaling cascade. Adv Exp Med Biol 720:163-70
Hei, Tom K; Ballas, Leslie K; Brenner, David J et al. (2009) Advances in radiobiological studies using a microbeam. J Radiat Res 50 Suppl A:A7-A12
Chai, Yunfei; Hei, Tom K (2008) Radiation Induced Bystander Effect in vivo. Acta Med Nagasaki 53:S65-S69
Brenner, David J (2008) The linear-quadratic model is an appropriate methodology for determining isoeffective doses at large doses per fraction. Semin Radiat Oncol 18:234-9
Hei, Tom K; Zhou, Hongning; Ivanov, Vladimir N et al. (2008) Mechanism of radiation-induced bystander effects: a unifying model. J Pharm Pharmacol 60:943-50
Ponnaiya, Brian; Jenkins-Baker, Gloria; Randers-Pherson, Gerhard et al. (2007) Quantifying a bystander response following microbeam irradiation using single-cell RT-PCR analyses. Exp Hematol 35:64-8
Hei, Tom K (2006) Cyclooxygenase-2 as a signaling molecule in radiation-induced bystander effect. Mol Carcinog 45:455-60

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