Abstract

The identification of a chromosomal biomarker for high LET radiation would facilitate the identification of individuals exposed not only to domestic and occupational radon which may be responsible for specific health effects, but also to fission neutrons. Studies have suggested that high LET radiation produces a low ratio [F] of inter- to intrachromosome interarm exchange aberrations compared with low LET radiation. Using multicolor fluorescence in situ hybridization [FISH] painting we analyzed the frequency of inter- and intrachromosome exchanges.Normal human fibroblasts in the G1 phase and G0 human lymphocytes were exposed to 2-8 Gy of 0.6 Gy /hr 137 Cs gamma -rays , 0.2-0.8 Gy of 430 keV neutrons , or 0.2-0.8 Gy 65 keV neutrons. The F-ratio was found to be dose dependent and confounded by the high proportion of complex exchanges observed after high LET radiation. A higher frequency of complex exchanges was observed after neutron radiation [22-30%] compared with LDR gamma -rays [8-22%]. The frequency of complex exchanges was found to be higher in lymphocytes than fibroblasts , particularly after high LET radiation. These studies encourage consideration of this approach for the analysis of latent viable chromosomal changes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR011623-05
Application #
6346376
Study Section
Project Start
2000-09-01
Project End
2001-08-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2000
Total Cost
$63,695
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032

  Publications

Chai, Y; Calaf, G M; Zhou, H et al. (2013) Radiation induced COX-2 expression and mutagenesis at non-targeted lung tissues of gpt delta transgenic mice. Br J Cancer 108:91-8
Chai, Y; Lam, R K K; Calaf, G M et al. (2013) Radiation-induced non-targeted response in vivo: role of the TGFýý-TGFBR1-COX-2 signalling pathway. Br J Cancer 108:1106-12
Hu, Burong; Grabham, Peter; Nie, Jing et al. (2012) Intrachromosomal changes and genomic instability in site-specific microbeam-irradiated and bystander human-hamster hybrid cells. Radiat Res 177:25-34
Hei, Tom K; Zhao, Yongliang; Zhou, Hongning et al. (2011) Mechanism of radiation carcinogenesis: role of the TGFBI gene and the inflammatory signaling cascade. Adv Exp Med Biol 720:163-70
Hei, Tom K; Ballas, Leslie K; Brenner, David J et al. (2009) Advances in radiobiological studies using a microbeam. J Radiat Res 50 Suppl A:A7-A12
Chai, Yunfei; Hei, Tom K (2008) Radiation Induced Bystander Effect in vivo. Acta Med Nagasaki 53:S65-S69
Brenner, David J (2008) The linear-quadratic model is an appropriate methodology for determining isoeffective doses at large doses per fraction. Semin Radiat Oncol 18:234-9
Hei, Tom K; Zhou, Hongning; Ivanov, Vladimir N et al. (2008) Mechanism of radiation-induced bystander effects: a unifying model. J Pharm Pharmacol 60:943-50
Ponnaiya, Brian; Jenkins-Baker, Gloria; Randers-Pherson, Gerhard et al. (2007) Quantifying a bystander response following microbeam irradiation using single-cell RT-PCR analyses. Exp Hematol 35:64-8
Hei, Tom K (2006) Cyclooxygenase-2 as a signaling molecule in radiation-induced bystander effect. Mol Carcinog 45:455-60

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