This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A little studied ubiquitin-like system is required for filamentous growth and several other processes in yeast cells. In particular, the ubiquitin-like modifier Urm1 and the E1 enzyme Uba4 are required for cell elongation during filamentous growth. Mutants lacking either of these proteins are also sensitive to some organic oxidizing agents and to rapamycin. Putative E2 and E3 enzymes for the 'urmylation' pathway have not been identified. Likewise, cellular proteins that recognize a target that has been urmylated have not been identified. We propose a two-hybrid screen using a Urm1-Gal4 BD construct to identify such proteins.
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