This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DNA damage can be deleterious to cell survival and cells have adopted multiple strategies to bypass or physically remove certain specific types of DNA lesions. Also, cells can slow down their progression through different cell cycle phases to provide time for repair to occur and prevent mutation mutations from being propagated. Sequential phosphorylation event plays a key role in activating the DNA repair machinery. In most of the cases, individual signaling network for DNA repair has been well characterized, still little is known about the global regulation of kinase activities and other ATP-binding proteins involved in the process. Heterogeneity and low abundance make the analysis difficult. With an ATP-binding protein enrichment tool, we could selectively enrich a major portion of the kinases and other ATP-binding proteins in budding yeast S. cerevisiae. Combining kinase enrichment tool, peptide chromatography and tandem mass spectrometry, we are able to quantitatively assess changes in kinase expression level. The method developed could well be applied to higher organisms. Small kinase inhibitor molecules are frequently used to dissect signaling pathways or to counteract oncogenic events. However, a functional compensation is established by a homolog kinase of the same family. The phenomenon is true to many examples with serine/threonine kinase families. A global view of kinase expression level at certain cell event would help a better understanding of kinase regulation and drug design.
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