This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are studying the physical and functional links between S. cerevisiae mitotic spindle checkpoint proteins and kinetochore proteins. Kinetochore association of checkpoint protein complexes is required for checkpoint function and checkpoint proteins perform important roles in generating proper kinetochore structure and function. By tandem affinity purification we have purified Bub3p, a checkpoint protein that binds the kinetochore and associates with the Bub1p kinase plus several unidentified proteins. Mass spectrometric analysis of the Bub3p complex should reveal checkpoint-kinetochore protein interactions and will be an important starting point for further investigations of their relationships.
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