EXCEED THE SPACE PROVIDED. The overall goal of the Research Resource Multiscale Modeling Tools for Structural Biology is the development and integration of modeling tools for the exploration of multi-resolution models in structural biology. Problems in structural biology require researchers to move between models of low-resolution and detailed atomic models to fully explore and exploit experimental information. Our resource efforts through core and collaborative research programs are focused on the development of new and integrated approaches to multiscale modeling in four primary focus areas: ? Modeling of large-scale assemblies of nucleic acids and proteins with nucleic acids, including the facile transitioning between models at full atomic resolution and low-resolution representations. ? Protein structure prediction and refinement using hybrid methods combining lattice-based Monte Carlo algorithms with force field based methods, including the requisite tools. Computational infrastructure and data bases that facilitate the development of prediction pipelines. ? The development of algorithms, methods and tools to explore large-scale motions that occur during a virus life cycle, i.e. assembly, maturation and infectivity, as well as a web-based infrastructure and data repository for virus structures and derived properties. ? Novel methods and tools to be used by structural biologists employing cryo-EMto build and refine multiscale structural models. Particular focus will be given to the flexible refinement of atomic-level structures into low-resolution data using elastic network normal mode methods. An additional core research effort ties these threads together through the development and distribution of computer codes to make such simulations readily accessible to the scientific community at large. We complement these developments by: ? collaborations that challenge and direct our Core research efforts ? dissemination and distribution of algorithmic and methodological developments through the established distribution mechanisms employed for the widely distributed Amber and CHARMM molecular modeling packages ? continued development of the VIPER """"""""web-base"""""""" for structural virology ? training of biomedical researchers through research and training workshops

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR012255-10
Application #
7121189
Study Section
Special Emphasis Panel (ZRG1-BCMB-E (40))
Program Officer
Brazhnik, Olga
Project Start
1997-08-15
Project End
2010-08-31
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
10
Fiscal Year
2006
Total Cost
$1,104,685
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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Bruno, Paul A; Morriss-Andrews, Alex; Henderson, Andrew R et al. (2016) A Synthetic Loop Replacement Peptide That Blocks Canonical NF-?B Signaling. Angew Chem Int Ed Engl 55:14997-15001
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Cheng, Shanshan; Brooks 3rd, Charles L (2015) Protein-Protein Interfaces in Viral Capsids Are Structurally Unique. J Mol Biol 427:3613-3624
Carrillo-Tripp, Mauricio; Montiel-García, Daniel Jorge; Brooks 3rd, Charles L et al. (2015) CapsidMaps: protein-protein interaction pattern discovery platform for the structural analysis of virus capsids using Google Maps. J Struct Biol 190:47-55
Ahlstrom, Logan S; Law, Sean M; Dickson, Alex et al. (2015) Multiscale modeling of a conditionally disordered pH-sensing chaperone. J Mol Biol 427:1670-80
Mustoe, Anthony M; Brooks, Charles L; Al-Hashimi, Hashim M (2014) Hierarchy of RNA functional dynamics. Annu Rev Biochem 83:441-66
Nobrega, R Paul; Arora, Karunesh; Kathuria, Sagar V et al. (2014) Modulation of frustration in folding by sequence permutation. Proc Natl Acad Sci U S A 111:10562-7
Arthur, Evan J; King, John T; Kubarych, Kevin J et al. (2014) Heterogeneous preferential solvation of water and trifluoroethanol in homologous lysozymes. J Phys Chem B 118:8118-27

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