The work done at NSLS bearalines is a segment of a complete PX project that often is minor in time, but crucial in its importance. Typical experimenters come to BNL only for brief periods, say 3-4 days, and rarely more than 2-3 times each year. During visits, they are expected to keep the apparatus running around the clock, and they try to use their precious beam-time allocation efficiently. There are several consequences of these visits being short, intense, and widely spaced, and of these measurements taking a short time in the PX process. One is that the experimenters rarely become completely familiar with the apparatus and the software that drives it. Secondly, they often are working with little sleep under stressful conditions. Finally, a team of three or four experimenters often will include one or two who are not really experienced crystallographers. They simply are not able to make all the decisions that might need to be made during data collection. A major goal of those of us who operate these facilities is continually to improve the interface between the user and the instruments. This interface includes both the physical presentation of the apparatus and the appearance of the software that controls it. There are several aspects to this work. Firstly, one must meet continuing advances in the quality of the experimental hardware. Of course computers must be continually upgraded, but the measurement apparatus itself seems to have an obsolescence lifetime of about three years. When apparatus is replaced, the control mechanisms and software also must be upgraded or replaced, along with the documentation and training program. We take very seriously the role that good instrument-control and data-reduction software can play in making use of the facilities reliable and as easy as possible. To that end, we employ a full-time programmer (John Skinner). This allows us easily to respond to new experimental situations (new equipment or software) and also to reply to users' suggestions or complaints. The beamline software we present to our users now has several aspects of the experiment integrated into one package, is fairly easy for the users to learn to use, and is filled with checks to prevent mis-steps and to guide the user in making measurements.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR012408-05
Application #
6491533
Study Section
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
5
Fiscal Year
2001
Total Cost
Indirect Cost
Name
Brookhaven National Laboratory
Department
Type
DUNS #
027579460
City
Upton
State
NY
Country
United States
Zip Code
11973
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Sui, Xuewu; Farquhar, Erik R; Hill, Hannah E et al. (2018) Preparation and characterization of metal-substituted carotenoid cleavage oxygenases. J Biol Inorg Chem 23:887-901
Fuller, Franklin D; Gul, Sheraz; Chatterjee, Ruchira et al. (2017) Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. Nat Methods 14:443-449
Wangkanont, Kittikhun; Winton, Valerie J; Forest, Katrina T et al. (2017) Conformational Control of UDP-Galactopyranose Mutase Inhibition. Biochemistry 56:3983-3992
VanderLinden, Ryan T; Hemmis, Casey W; Yao, Tingting et al. (2017) Structure and energetics of pairwise interactions between proteasome subunits RPN2, RPN13, and ubiquitin clarify a substrate recruitment mechanism. J Biol Chem 292:9493-9504
Song, Lingshuang; Yang, Lin; Meng, Jie et al. (2017) Thermodynamics of Hydrophobic Amino Acids in Solution: A Combined Experimental-Computational Study. J Phys Chem Lett 8:347-351
Orlova, Natalia; Gerding, Matthew; Ivashkiv, Olha et al. (2017) The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators. Nucleic Acids Res 45:3724-3737
Firestone, Ross S; Cameron, Scott A; Karp, Jerome M et al. (2017) Heat Capacity Changes for Transition-State Analogue Binding and Catalysis with Human 5'-Methylthioadenosine Phosphorylase. ACS Chem Biol 12:464-473
Tajima, Nami; Karakas, Erkan; Grant, Timothy et al. (2016) Activation of NMDA receptors and the mechanism of inhibition by ifenprodil. Nature 534:63-8
Ericson, Daniel L; Yin, Xingyu; Scalia, Alexander et al. (2016) Acoustic Methods to Monitor Protein Crystallization and to Detect Protein Crystals in Suspensions of Agarose and Lipidic Cubic Phase. J Lab Autom 21:107-14

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