This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.uvsY is the prototypical member of a family of proteins that prepares single-stranded DNA for recombination by dislodging gp32 and recruiting the recombinase uvsX. The structural mechanism by which this is accomplished remains to be determined. To begin to understand how uvsY interacts with DNA, we have grown crystals of the protein alone and in complex with ssDNA for structure determination by crystallographic methods.Genetic recombination is the engine that drives organismic diversity and evolution. This complex process involves the exchange of segments of DNA between similar but not identical strands. Little is known about the structural mechanism underlying this process, or the assembly of the recombination machinery. Since bacteria and cancer cells use recombination to generate resistance to therapeutic agents, the ability to inhibit this process pharmaceutically may impede the acquisition of such resistance.
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