This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The nuclear pore complex (NPC) is a large macromolecular structure composed of at least 30 different protein subunits. The most obvious roles of the NPC is being the sole gateway for nucleocytoplasmic exchange of macromolecules, either through passive diffusion or facilitated transported. The NPC consists of three distinct architectural structures: the central framework, the cytoplasmic filaments and the nuclear basket. The central framework is believed to form the channel that connects the cytoplasm and inner nucleus. The NPC contains an eight fold symmetry. Due do this symmetry, most nucleoporin should be present at a copy number of eight or a multiple of eight. Most of the nucleoporins are located symmetrically on both sides of the NPC, whereas only a few are positioned at either the nuclear or the cytoplasmic face. For a complete picture of the NPC architecture a high resolution three dimensional structure would be required. However, it is not feasible to obtain such a map on the complete structure at once. A collaborative approach taken by the Blobel lab to build such a model is underway. I have cocystalized two components of the nuclearpore complex, Sec13 and a fragment of Nup96.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR012408-12
Application #
7726220
Study Section
Special Emphasis Panel (ZRG1-BCMB-R (40))
Project Start
2008-09-18
Project End
2009-06-30
Budget Start
2008-09-18
Budget End
2009-06-30
Support Year
12
Fiscal Year
2008
Total Cost
$8,536
Indirect Cost
Name
Brookhaven National Laboratory
Department
Type
DUNS #
027579460
City
Upton
State
NY
Country
United States
Zip Code
11973
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