Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The actin cytoskeleton near the protruding cell edge consists of two kinetically, kinematically, molecularly, and materially distinct networks. Only one of the two, which we refer to as the lamellipodium, has the properties of an Arp2/3-controlled, dendritically branched and treadmilling network. The second network, referred to as the lamella, does contain Arp2/3 as well, but its polymerization is largely independent of Arp2/3 activity. We hypothesize that the assembly of F-actin in the lamella is mediated by formins and tropomyosin.
The aim of this project is to implement a Virtual Cell (VC) model of F-actin network assembly at the leading edge to predict the differential kinetics of simultaneous Arp2/3- and formin-mediated lamellipodium and lamella formation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR013186-11
Application #
7722730
Study Section
Special Emphasis Panel (ZRG1-CB-L (40))
Project Start
2008-08-01
Project End
2009-04-30
Budget Start
2008-08-01
Budget End
2009-04-30
Support Year
11
Fiscal Year
2008
Total Cost
$27,639
Indirect Cost

  Institution

Name
University of Connecticut
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030

  Publications

Ron, Amit; Azeloglu, Evren U; Calizo, Rhodora C et al. (2017) Cell shape information is transduced through tension-independent mechanisms. Nat Commun 8:2145
Schaff, James C; Gao, Fei; Li, Ye et al. (2016) Numerical Approach to Spatial Deterministic-Stochastic Models Arising in Cell Biology. PLoS Comput Biol 12:e1005236
Semenova, Irina; Ikeda, Kazuho; Resaul, Karim et al. (2014) Regulation of microtubule-based transport by MAP4. Mol Biol Cell 25:3119-32
Novak, Igor L; Slepchenko, Boris M (2014) A conservative algorithm for parabolic problems in domains with moving boundaries. J Comput Phys 270:203-213
Michalski, Paul J (2014) First demonstration of bistability in CaMKII, a memory-related kinase. Biophys J 106:1233-5
Azeloglu, Evren U; Hardy, Simon V; Eungdamrong, Narat John et al. (2014) Interconnected network motifs control podocyte morphology and kidney function. Sci Signal 7:ra12
Dickson, Eamonn J; Falkenburger, Björn H; Hille, Bertil (2013) Quantitative properties and receptor reserve of the IP(3) and calcium branch of G(q)-coupled receptor signaling. J Gen Physiol 141:521-35
Michalski, P J (2013) The delicate bistability of CaMKII. Biophys J 105:794-806
Falkenburger, Björn H; Dickson, Eamonn J; Hille, Bertil (2013) Quantitative properties and receptor reserve of the DAG and PKC branch of G(q)-coupled receptor signaling. J Gen Physiol 141:537-55
Ditlev, Jonathon A; Mayer, Bruce J; Loew, Leslie M (2013) There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton. Biophys J 104:520-32

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