This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Driven by imaging data from existing and planned imaging experiments in our laboratory and through collaborations within the Cell Migration Consortium, we are building a spatial model of PI3K/RFG co-regulation at the whole-cell level. A phenomenological model of signaling-protrusion coupling will be constructed, guided by analysis of live-cell imaging experiments. To the extent possible, we will develop the model, or modules thereof, in the Virtual Cell environment. The Haugh group has previously made use of the new membrane diffusion capabilities of VCell in our work on gradient sensing, and these models are publicly available to other VCell users. Cell mechanical modeling capabilities, currently under development by the VCell team in collaboration with the CMC modeling initiative, will be utilized in this project as they become available. These will depend on the elliptic solver planned for the next NRCAM project period. Incorporation of mechanics into the VCell framework will then be facilitated by the newly proposed multiphysics layer and plug-in architecture.
| Ron, Amit; Azeloglu, Evren U; Calizo, Rhodora C et al. (2017) Cell shape information is transduced through tension-independent mechanisms. Nat Commun 8:2145 |
| Schaff, James C; Gao, Fei; Li, Ye et al. (2016) Numerical Approach to Spatial Deterministic-Stochastic Models Arising in Cell Biology. PLoS Comput Biol 12:e1005236 |
| Semenova, Irina; Ikeda, Kazuho; Resaul, Karim et al. (2014) Regulation of microtubule-based transport by MAP4. Mol Biol Cell 25:3119-32 |
| Novak, Igor L; Slepchenko, Boris M (2014) A conservative algorithm for parabolic problems in domains with moving boundaries. J Comput Phys 270:203-213 |
| Michalski, Paul J (2014) First demonstration of bistability in CaMKII, a memory-related kinase. Biophys J 106:1233-5 |
| Azeloglu, Evren U; Hardy, Simon V; Eungdamrong, Narat John et al. (2014) Interconnected network motifs control podocyte morphology and kidney function. Sci Signal 7:ra12 |
| Ditlev, Jonathon A; Mayer, Bruce J; Loew, Leslie M (2013) There is more than one way to model an elephant. Experiment-driven modeling of the actin cytoskeleton. Biophys J 104:520-32 |
| Acker, Corey D; Loew, Leslie M (2013) Characterization of voltage-sensitive dyes in living cells using two-photon excitation. Methods Mol Biol 995:147-60 |
| Loew, Leslie M; Hell, Stefan W (2013) Superresolving dendritic spines. Biophys J 104:741-3 |
| Blasius, T Lynne; Reed, Nathan; Slepchenko, Boris M et al. (2013) Recycling of kinesin-1 motors by diffusion after transport. PLoS One 8:e76081 |
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