This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microimaging and spectroscopy of very small samples requires the development of small rf coils in order to maximize the signal obtained. In accordance with Aim #1 of Core 2, we have been constructing a range of solenoidal coils for the 750MHz instrument for a range of sample ranging from single cells, alginate beads, spinal cords, muscle fibers and mouse brains, and this work has continued this year. This year has required the incorporation of perfusion with the probes, particularly for studies with our collaborators on alginate beads and islets. Additionally P31 coils have been constructed for studies of isolated muscle fibers. We have also constructed coils for operation at 13C and O17 at 4.7T, 11.1T and on the 17.6T systems. The 13C coils support studies on spectroscopy of rat tumors with Dr Mike Gamscik (Duke). The O17 is being developed in collaboration with Wei Chen for monitoring tissue oxygen consumption rate. The SNR is critical in these studies, and we are pinning our best hopes on the 17.6T magnet. To date, we have constructed a compete rf probe and probe body with an O17 coil, a separate butterfly 1H coil, and animal maintenance and monitoring. Using this probe we have obtained the first O17 CSI data and O17 images of a live mouse brain.
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