This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Under the auspices of the NIH supported Brain Molecular Anatomy Project, we are in the process of generating an anatomical 3D template of the C57BL6/J mouse brain using magnetic resonance microscopy (MRM). Major challenges involved in anatomical atlas generation is defining MR contrast and imaging parameters suitable for a multitude of micro-anatomical structures, and obtaining the highest spatial resolutions possible. Thus signal to noise is crucial. Consequently we will use the highest magnetic fields available, namely the 750MHz instrument at UF, to generate the mouse brain atlas. Two main applications have been pursued using these coils. First, the effects of ethanol toxicity have been explored, resulting in significant change sin brain structure. Secondly we have been exploring ways to determine the exact morphological features in the brain tissues. In one approach, microinjections of chemicals known to destroy specific cell layers has been explored. We are continuing data collection and analysis for these studies.
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