This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.ACERT is scheduled for installation of a Cryogenic, Ltd. 9T cryogen-free magnet and low-temperature insert in late August, 2007. This schedule represents a 3-month delay from the original installation date. For impending step-frequency 95 GHz DEER experiments on protein systems in our existing 6T Oxford magnet, we have therefore proceeded with specification and design of a significant interim upgrade to our earlier JOMO-I temperature control system. The Controlled Temperature Insert (CTI) upgrade that we are presently assembling consists of an added closed-loop gas pre-heater section, construction of a low time-constant temperature control heater within the existing 'ambienstat' insert structure and temperature monitoring of one or both of the vacuum-retaining warm bore 'O'-ring seals. The existing Lakeshore model 331 temperature controller, with input from an appropriate sensor at the sample, will be retained as the primary temperature regulating system. Temperature control down to 100 K at 0.1 K stability is expected.All components for the interim CTI upgrade have been acquired, and the insert structure has been modified to accept a heater/armature assembly in a position of close proximity (7 cm) to the sample. A rack chassis with front panel Omega controller/monitors and heater power driver circuitry for preheater regulation and O-ring seals temperature monitoring (and, optionally, control) is currently under construction. We intend to complete construction and debug of the rackmount CTI preheater control system within a few weeks, at which time the controller will be installed in the 95 GHz magnet in concert with the revised ambienstat and cryostat O-ring seal monitors.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR016292-08
Application #
7723967
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2008-09-01
Project End
2009-08-31
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
8
Fiscal Year
2008
Total Cost
$2,595
Indirect Cost
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Jain, Rinku; Vanamee, Eva S; Dzikovski, Boris G et al. (2014) An iron-sulfur cluster in the polymerase domain of yeast DNA polymerase ?. J Mol Biol 426:301-8
Pratt, Ashley J; Shin, David S; Merz, Gregory E et al. (2014) Aggregation propensities of superoxide dismutase G93 hotspot mutants mirror ALS clinical phenotypes. Proc Natl Acad Sci U S A 111:E4568-76
Georgieva, Elka R; Borbat, Peter P; Ginter, Christopher et al. (2013) Conformational ensemble of the sodium-coupled aspartate transporter. Nat Struct Mol Biol 20:215-21
Airola, Michael V; Sukomon, Nattakan; Samanta, Dipanjan et al. (2013) HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors. PLoS Biol 11:e1001479
Airola, Michael V; Huh, Doowon; Sukomon, Nattakan et al. (2013) Architecture of the soluble receptor Aer2 indicates an in-line mechanism for PAS and HAMP domain signaling. J Mol Biol 425:886-901
Sun, Yan; Zhang, Ziwei; Grigoryants, Vladimir M et al. (2012) The internal dynamics of mini c TAR DNA probed by electron paramagnetic resonance of nitroxide spin-labels at the lower stem, the loop, and the bulge. Biochemistry 51:8530-41
Smith, Andrew K; Freed, Jack H (2012) Dynamics and ordering of lipid spin-labels along the coexistence curve of two membrane phases: an ESR study. Chem Phys Lipids 165:348-61
Yu, Renyuan Pony; Darmon, Jonathan M; Hoyt, Jordan M et al. (2012) High-Activity Iron Catalysts for the Hydrogenation of Hindered, Unfunctionalized Alkenes. ACS Catal 2:1760-1764
Gaffney, Betty J; Bradshaw, Miles D; Frausto, Stephen D et al. (2012) Locating a lipid at the portal to the lipoxygenase active site. Biophys J 103:2134-44
Dzikovski, Boris; Tipikin, Dmitriy; Freed, Jack (2012) Conformational distributions and hydrogen bonding in gel and frozen lipid bilayers: a high frequency spin-label ESR study. J Phys Chem B 116:6694-706

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