This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.In all eukaryotic cells, 26S proteasome catalyzes most intracellular protein degradation in an ATP dependent manner. The proteasome is composed of a 20S protease core particle (CP) sandwiched between two 19S regulatory complexes (RP). The proteasomal ATPases recognize the substrates targeted for the degradations, unfold globular substrates, induce gate-opening in the 20S, and facilitate translocation of the unfolded substrate into 20S CP for degradation. We are using single particle cryoEM together with other biochemical methods to study the mechanism of gate-opening in the 20S CP induced by the ATPases.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR017573-07
Application #
7723579
Study Section
Special Emphasis Panel (ZRG1-CB-B (40))
Project Start
2008-08-01
Project End
2009-04-30
Budget Start
2008-08-01
Budget End
2009-04-30
Support Year
7
Fiscal Year
2008
Total Cost
$6,310
Indirect Cost
Name
Scripps Research Institute
Department
Type
DUNS #
781613492
City
La Jolla
State
CA
Country
United States
Zip Code
92037
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