This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Reactive oxygen species (ROS) are required for mounting an effective immune response, yet ROS also produce detrimental oxidative modifications to host proteins. Previously we demonstrated that ROS induce oxidative modifications to host proteins and induce repair processes (1). Thus, the identification of changes in protein abundance as well as modifications during inflammation (e.g. aging or infection), will further our understanding of how ROS contribute to disease processes. In addition, characterization of changes in protein complexes will aid in the identification of vital pathways for targeted therapeutic intervention. Our ultimate goals are to identify changes in cellular process and the proteome based upon inflammation, determine oxidative modifications to proteins due to ROS, and determine alterations in protein complexes using high resolution separations and high performance mass spectrometry. These findings will be instrumental in defining the underlying cause of inflammatory disease and associated pathologies with the end goal of development of therapeutic interventions (e.g. pharmacological therapeutics) to curb disease processes.
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