Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.In yeast cells, endocytosis is entirely dependent upon the actin cytoskeleton. A major current area of our research is to elucidate the endocytic roles of actin. We have isolated numerous mutants that block endocytosis in yeast at discrete steps in the process, and we tagged some 20-30 endocytic and cytoskeletal proteins with GFP. We will use x-ray tomography to fully elucidate the spatial organization of the endocytic pathway and to relate this pathway with a variety of other cellular features. We will determine how these features change through the cell cycle in response to endocytic induction of the Ste2p receptor protein endocytosis and in response to mutation of specific endocytic and cytoskeletal proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR019664-05
Application #
7722171
Study Section
Special Emphasis Panel (ZRG1-F05 (40))
Project Start
2008-05-01
Project End
2009-04-30
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
5
Fiscal Year
2008
Total Cost
$35,323
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

McHugh, Emma; Batinovic, Steven; Hanssen, Eric et al. (2015) A repeat sequence domain of the ring-exported protein-1 of Plasmodium falciparum controls export machinery architecture and virulence protein trafficking. Mol Microbiol 98:1101-14
Do, Myan; Isaacson, Samuel A; McDermott, Gerry et al. (2015) Imaging and characterizing cells using tomography. Arch Biochem Biophys 581:111-21
Dasgupta, Sabyasachi; Auth, Thorsten; Gov, Nir S et al. (2014) Membrane-wrapping contributions to malaria parasite invasion of the human erythrocyte. Biophys J 107:43-54
Le Gros, Mark A; McDermott, Gerry; Cinquin, Bertrand P et al. (2014) Biological soft X-ray tomography on beamline 2.1 at the Advanced Light Source. J Synchrotron Radiat 21:1370-7
Smith, Elizabeth A; Cinquin, Bertrand P; Do, Myan et al. (2014) Correlative cryogenic tomography of cells using light and soft x-rays. Ultramicroscopy 143:33-40
Hanssen, Eric; Dekiwadia, Chaitali; Riglar, David T et al. (2013) Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion. Cell Microbiol 15:1457-72
Smith, Elizabeth A; Cinquin, Bertrand P; McDermott, Gerry et al. (2013) Correlative microscopy methods that maximize specimen fidelity and data completeness, and improve molecular localization capabilities. J Struct Biol 184:12-20
Parkinson, Dilworth Y; Epperly, Lindsay R; McDermott, Gerry et al. (2013) Nanoimaging cells using soft X-ray tomography. Methods Mol Biol 950:457-81
Isaacson, Samuel A; Larabell, Carolyn A; Le Gros, Mark A et al. (2013) The influence of spatial variation in chromatin density determined by X-ray tomograms on the time to find DNA binding sites. Bull Math Biol 75:2093-117
McDermott, Gerry; Le Gros, Mark A; Larabell, Carolyn A (2012) Visualizing cell architecture and molecular location using soft x-ray tomography and correlated cryo-light microscopy. Annu Rev Phys Chem 63:225-39

Showing the most recent 10 out of 29 publications