Analytical Core Polychlorinated biphenyls (PCBs) are important Superfund chemicals that are known human carcinogens also associated with neurological and metabolic disruption. The Analytical Core (AC) provides analytical services to the ISRP to facilitate high-quality research on PCBs, including data, standard operating procedures, and peer- reviewed publications that are standardized, efficient, and comparable across all ISRP matrices and projects. The AC provides instrumentation and facilities, expert staff, training, data sharing platforms, and support for method development. The AC will continue to accomplish these activities through five Specific Aims:
Aim 1. Maintain analytical quality assurance (QA) standards and protocols. Accurate, precise, representative, reproducible, and comparable sample analysis is absolutely essential for all five ISRP projects and is a key AC objective. The exceptional rigor of the QA protocol for PCB analysis provides a critical foundation for discoveries by the ISRP projects.
Aim 2. Facilitate high-throughput analysis in complex biotic and abiotic matrices. The AC supports ISRP projects by extracting and analyzing samples for all 209 PCBs and 72 OH- PCBs as MeO-PCBs in complex matrices, including: air samples collected on quartz filters, XAD resin, and polyurethane foam; sediment, water, and pore water samples; human serum; laboratory animal tissues; plant tissues; soils; microbial cultures; and polymers, paints, and pigments. To support new research directions within the ISRP, we will expand our capabilities to include analyses of food matrices and the ability to quantify all 837 possible mono-hydroxylated PCBs.
Aim 3. Provide prioritized training and access to AC facilities. The AC, in collaboration with the Research Experience and Training Coordination Core, provides prioritized, standardized training in AC facilities and methods to graduate student and postdoc trainees, faculty, and staff from all ISRP projects. Trainees also receive assistance in method development and use of instrumentation.
Aim 4. Support method development for complex matrices. The AC, in collaboration with ISRP projects, will continue to develop, maintain, and update methods for high-throughput PCB and OH-PCB extraction and analysis in biotic and abiotic samples. Separate but comparable methods are maintained across matrices to facilitate integrated data analysis.
Aim 5. Maintain analytical infrastructure. The AC maintains critical infrastructure, including high-use analytical instruments, a sample database, and archives such as standard solutions, samples, and sample extracts. The AC, in collaboration with the Data Management and Analysis Core, will provide oversight to make data findable, accessible, interoperable, and reusable (FAIR) by ISRP investigators. Overall, the AC is a fully-integrated support core that assists ISRP projects to meet all four SRP mandates requiring methods for identifying human health hazards of superfund chemicals, detection, assessment of exposure risk, and remediation.

Public Health Relevance

Analytical Core The Analytical Core (AC) provides analytical services to the Iowa Superfund Research Program to facilitate high-quality research on polychlorinated biphenyls (PCBs). The AC provides instrumentation and facilities, quality assurance standards and protocols, expert staff, training, data sharing platforms, and support for method development.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Hazardous Substances Basic Research Grants Program (NIEHS) (P42)
Project #
2P42ES013661-15
Application #
9839862
Study Section
Special Emphasis Panel (ZES1)
Project Start
Project End
2025-01-31
Budget Start
2020-04-01
Budget End
2021-03-31
Support Year
15
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Hou, Xingwang; Yu, Miao; Liu, Aifeng et al. (2018) Biotransformation of tetrabromobisphenol A dimethyl ether back to tetrabromobisphenol A in whole pumpkin plants. Environ Pollut 241:331-338
Xiao, Xin; Chen, Baoliang; Chen, Zaiming et al. (2018) Insight into Multiple and Multilevel Structures of Biochars and Their Potential Environmental Applications: A Critical Review. Environ Sci Technol 52:5027-5047
Herkert, Nicholas J; Jahnke, Jacob C; Hornbuckle, Keri C (2018) Emissions of Tetrachlorobiphenyls (PCBs 47, 51, and 68) from Polymer Resin on Kitchen Cabinets as a Non-Aroclor Source to Residential Air. Environ Sci Technol 52:5154-5160
P?n?íková, Kate?ina; Svržková, Lucie; Strapá?ová, Simona et al. (2018) In vitro profiling of toxic effects of prominent environmental lower-chlorinated PCB congeners linked with endocrine disruption and tumor promotion. Environ Pollut 237:473-486
P?n?íková, Kate?ina; Brenerová, Petra; Svržková, Lucie et al. (2018) Atropisomers of 2,2',3,3',6,6'-hexachlorobiphenyl (PCB 136) exhibit stereoselective effects on activation of nuclear receptors in vitro. Environ Sci Pollut Res Int 25:16411-16419
Robertson, Larry W; Weber, Roland; Nakano, Takeshi et al. (2018) PCBs risk evaluation, environmental protection, and management: 50-year research and counting for elimination by 2028. Environ Sci Pollut Res Int 25:16269-16276
Klaren, William D; Vine, David; Vogt, Stefan et al. (2018) Spatial distribution of metals within the liver acinus and their perturbation by PCB126. Environ Sci Pollut Res Int 25:16427-16433
Tomsho, Kathryn S; Basra, Komal; Rubin, Staci M et al. (2018) Correction to: Community reporting of ambient air polychlorinated biphenyl concentrations near a Superfund site. Environ Sci Pollut Res Int 25:16401
Uwimana, Eric; Li, Xueshu; Lehmler, Hans-Joachim (2018) Human Liver Microsomes Atropselectively Metabolize 2,2',3,4',6-Pentachlorobiphenyl (PCB 91) to a 1,2-Shift Product as the Major Metabolite. Environ Sci Technol 52:6000-6008
Herkert, Nicholas J; Hornbuckle, Keri C (2018) Effects of room airflow on accurate determination of PUF-PAS sampling rates in the indoor environment. Environ Sci Process Impacts 20:757-766

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