Abstract

In order to understand some of the functions of neurons in the basal forebrain in magnocellular complex (BFMC), it is essential to identify the sources and transmitter specificities of afferents to this system. Using axonal tracing and transmitter immunocytochemistry (ICC), Pilot 4 is designed to characterize, at the light and ultrastructural levels, neural systems that innervate BFMC cholinergic neurons in rodents. The anterograde tracer Phaseolus vulagaris leukoagglutinin (PHA-L) will be iontophoresed into regions known to be labeled following injections of retrograde tracer into subdivisions of the BFMC, and dual-color ICC will be employed to identify specific interactions between PHA-L-labeled boutons and cholinergic neurons. Monoaminergic, cholinergic, gamma- aminobutyric acid (GABA)ergic, and peptidergic transmitter-specific interactions with cholinergic neurons of the BFMC will be identified at the electron-microscopic level using double-label ICC> These studies will provide new insights concerning afferents that modulate cholinergic BFMC neurons in rodents and are essential for similar investigations of these afferents in primates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Specialized Center (P50)
Project #
5P50AG005146-10
Application #
3790129
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Johns Hopkins University
Department
Type
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218

  Publications

Ayhan, Fatma; Perez, Barbara A; Shorrock, Hannah K et al. (2018) SCA8 RAN polySer protein preferentially accumulates in white matter regions and is regulated by eIF3F. EMBO J 37:
Sathe, Gajanan; Na, Chan Hyun; Renuse, Santosh et al. (2018) Phosphotyrosine profiling of human cerebrospinal fluid. Clin Proteomics 15:29
Chen, Lin; Wei, Zhiliang; Chan, Kannie W Y et al. (2018) Protein aggregation linked to Alzheimer's disease revealed by saturation transfer MRI. Neuroimage 188:380-390
Gomez, Gabriela; Beason-Held, Lori L; Bilgel, Murat et al. (2018) Metabolic Syndrome and Amyloid Accumulation in the Aging Brain. J Alzheimers Dis 65:629-639
Burke, Shanna L; Cadet, Tamara; Maddux, Marlaina (2018) Chronic Health Illnesses as Predictors of Mild Cognitive Impairment Among African American Older Adults. J Natl Med Assoc 110:314-325
Martin, Lee J; Chang, Qing (2018) DNA Damage Response and Repair, DNA Methylation, and Cell Death in Human Neurons and Experimental Animal Neurons Are Different. J Neuropathol Exp Neurol 77:636-655
Kim, Jeongyong; Bandeen-Roche, Karen (2018) Parametric estimation of association in bivariate failure-time data subject to competing risks: sensitivity to underlying assumptions. Lifetime Data Anal :
Bilgel, Murat; An, Yang; Helphrey, Jessica et al. (2018) Effects of amyloid pathology and neurodegeneration on cognitive change in cognitively normal adults. Brain :
Hadjichrysanthou, Christoforos; McRae-McKee, Kevin; Evans, Stephanie et al. (2018) Potential Factors Associated with Cognitive Improvement of Individuals Diagnosed with Mild Cognitive Impairment or Dementia in Longitudinal Studies. J Alzheimers Dis 66:587-600
Yasar, Sevil; Varma, Vijay R; Harris, Gregory C et al. (2018) Associations of Angiotensin Converting Enzyme-1 and Angiotensin II Blood Levels and Cognitive Function. J Alzheimers Dis 63:655-664

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