Abstract

This renewal contains the research projects of six investigators, plus an outreach clinical program. All of six projects investigate clinically relevant problems taking cellular and molecular biological approaches to solve them, on either human or experimental material. Two projects (Drs. Unanue and Loh) use murine experimental systems while the remaining four (Drs. Parker, Schwartz, Chaplin and Lublin) deal directly with human material. The blending of basic scientists and clinical investigators in this program should create a mutally beneficial situation. The project of Dr. Emil R. Unanue deals with cellular immunity to infection and the regulation of antigen presentation using an experimental model of a mouse mutant strain that lacks lymphocytes. Dr. Dennis Loh uses molecular biological approaches with transgenic mice to study T cell recognition and autoimmunity. Dr. Charles Parker tackles the molecular genetics of the allergic state. He and his associates examine for the distribution and possible genetic polymorphism of severaal genes claimed to be associated with allergies, plus examine the human IL-4 protein. Dr. Benjamin Schwartz uses cells transfected with HLA DR genes to study the recognition of influenza virus. He wants to examine how the class II histocompatibility molecules function in binding flu peptides, an issue relevant for our understanding of the anti-viral T cell response. Dr. David Chaplin examines the molecular genetics of systemic lupus erythematosus. His use of molecular biological technology may help us in understanding the genetic susceptibility of this frequent autoimmune disease. Dr. Douglas Lublin uses molecular biological approaches to study the decay accelerating factor of complement. His main interest is to study how the complement system is regulated during immune inflammation. All the projects, therefore, employ current molecular and cellular technologies to try to get to a basic understanding of the normal and abnormal immune response. Finally, Dr. James Wedner proposes an outreach program that will deal with the effect of an educational program on the long term clinical response of asthmatics.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center (P50)
Project #
5P50AI015322-12
Application #
3104991
Study Section
Allergy, Immunology, and Transplantation Research Committee (AITC)
Project Start
1978-09-01
Project End
1993-08-31
Budget Start
1989-09-01
Budget End
1990-08-31
Support Year
12
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130

  Publications

Flaskerud, J H; Nyamathi, A M (2000) Attaining gender and ethnic diversity in health intervention research: cultural responsiveness versus resource provision. ANS Adv Nurs Sci 22:15-Jan
Fisher Jr, E B; Strunk, R C; Sussman, L K et al. (1996) Acceptability and feasibility of a community approach to asthma management: the Neighborhood Asthma Coalition (NAC). J Asthma 33:367-83
Krishnan, B R; Jamry, I; Berg, D E et al. (1995) Construction of a genomic DNA 'feature map' by sequencing from nested deletions: application to the HLA class I region. Nucleic Acids Res 23:117-22
Krishnan, B R; Jamry, I; Chaplin, D D (1995) Feature mapping of the HLA class I region: localization of the POU5F1 and TCF19 genes. Genomics 30:53-8
Parker, C W; Huber, M G; Godt, S M (1995) Modulation of IL-4 production in murine spleen cells by prostaglandins. Cell Immunol 160:278-85
Nakayama, K; Nakayama, K; Negishi, I et al. (1994) Targeted disruption of Bcl-2 alpha beta in mice: occurrence of gray hair, polycystic kidney disease, and lymphocytopenia. Proc Natl Acad Sci U S A 91:3700-4
Gasser, D L; Sternberg, N L; Pierce, J C et al. (1994) P1 and cosmid clones define the organization of 280 kb of the mouse H-2 complex containing the Cps-1 and Hsp70 loci. Immunogenetics 39:48-55
Telen, M J; Rao, N; Udani, M et al. (1994) Molecular mapping of the Cromer blood group Cra and Tca epitopes of decay accelerating factor: toward the use of recombinant antigens in immunohematology. Blood 84:3205-11
Nygard, N R; Giacoletto, K S; Bono, C et al. (1994) Peptide binding to surface class II molecules is the major pathway of formation of immunogenic class II-peptide complexes for viable antigen presenting cells. J Immunol 152:1082-93
Nygard, N R; Schwartz, B D (1993) Infection and autoimmunity. Adv Intern Med 38:337-59

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