Abstract

The Tissue Culture Core will prepare, store and process RNA, protein and DNA from cultured dermal fibroblasts (and keratinocytes) from patients with scleroderma and controls for the studies proposed in Projects 1 and 3. Epstein-Barr virus (EBV) transformed lymphoblastoid cell lines from the Choctaw SSC cases, relatives and controls also are stored here and are regrown/processed for genomic DNA as needed. This core guarantees a perpetual source of DNA (and RNA and proteins from fibroblasts) from these well characterized study patients.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Specialized Center (P50)
Project #
5P50AR044888-06
Application #
6660903
Study Section
Project Start
2002-09-01
Project End
2003-08-31
Budget Start
Budget End
Support Year
6
Fiscal Year
2002
Total Cost
$183,721
Indirect Cost

  Institution

Name
University of Texas Health Science Center Houston
Department
Type
DUNS #
City
Houston
State
TX
Country
United States
Zip Code
77225

  Publications

McNearney, Terry A; Sallam, Hanaa S; Hunnicutt, Sonya E et al. (2013) Prolonged treatment with transcutaneous electrical nerve stimulation (TENS) modulates neuro-gastric motility and plasma levels of vasoactive intestinal peptide (VIP), motilin and interleukin-6 (IL-6) in systemic sclerosis. Clin Exp Rheumatol 31:140-50
Cockrill, Tonya; del Junco, Deborah J; Arnett, Frank C et al. (2010) Separate influences of birth order and gravidity/parity on the development of systemic sclerosis. Arthritis Care Res (Hoboken) 62:418-24
McNearney, Terry A; Sluka, K A; Ahn, C et al. (2010) Plasma endogenous enkephalin levels in early systemic sclerosis: clinical and laboratory associations. Clin Exp Rheumatol 28:S7-11
Sonnylal, Sonali; Shi-Wen, Xu; Leoni, Patricia et al. (2010) Selective expression of connective tissue growth factor in fibroblasts in vivo promotes systemic tissue fibrosis. Arthritis Rheum 62:1523-32
McNearney, Terry A; Hunnicutt, Sonya E; Fischbach, Michael et al. (2009) Perceived functioning has ethnic-specific associations in systemic sclerosis: another dimension of personalized medicine. J Rheumatol 36:2724-32
McNearney, T A; Sallam, H S; Hunnicutt, S E et al. (2009) Gastric slow waves, gastrointestinal symptoms and peptides in systemic sclerosis patients. Neurogastroenterol Motil 21:1269-e120
Assassi, Shervin; Del Junco, Deborah; Sutter, Kari et al. (2009) Clinical and genetic factors predictive of mortality in early systemic sclerosis. Arthritis Rheum 61:1403-11
Hunnicutt, Sonya E; Grady, James; McNearney, Terry A (2008) Complementary and alternative medicine use was associated with higher perceived physical and mental functioning in early systemic sclerosis. Explore (NY) 4:259-63
Assassi, Shervin; Arnett, Frank C; Reveille, John D et al. (2007) Clinical, immunologic, and genetic features of familial systemic sclerosis. Arthritis Rheum 56:2031-7
Zhao, Jinying; Boerwinkle, Eric; Xiong, Momiao (2007) An entropy-based genome-wide transmission/disequilibrium test. Hum Genet 121:357-67

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