Abstract

This a request to continue the Cell Sciences and Immunochemistry Core (CSIC). This Core has been established since July 1998 and has served the four projects well as outlined in the -01 application. The function of the Core remains to provide a central resource for timely, quality control, cost-effective services in immunochemistry, autoantibody assays, and cytokine measurements. In addition, the Core will provide advice and will carry out experiments to generate T-T and B-B hybridomas with desired specificities. The Core will develop new and improved procedures that will permit the investigators to ask novel and important questions as the research needs of the projects evolve. The CSIC will provide expertise and help in cloning new autoantigens from both kidney and salivary glands. The Director of the Core is assisted by the Co-Director and the Assistant Director. Four consultants with diverse expertise have been recruited. The services include recombinant antigens and peptides, antigen preparation, autoantibody assays, immunohistology, flow cytometry, and hybridoma generation. It is expected the CSIC will continue to serve all four projects as outlined in this competitive renewal.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Specialized Center (P50)
Project #
2P50AR045222-05
Application #
6663946
Study Section
Project Start
2002-09-27
Project End
2003-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
5
Fiscal Year
2002
Total Cost
$216,966
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Sung, Sun-Sang J; Ge, Yan; Dai, Chao et al. (2017) Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol 198:2589-2601
Dai, Chao; Deng, Yun; Quinlan, Aaron et al. (2014) Genetics of systemic lupus erythematosus: immune responses and end organ resistance to damage. Curr Opin Immunol 31:87-96
Ge, Yan; Jiang, Chao; Sung, Sun-Sang J et al. (2013) Cgnz1 allele confers kidney resistance to damage preventing progression of immune complex-mediated acute lupus glomerulonephritis. J Exp Med 210:2387-401
Lewis, Janet E; Fu, Shu Man; Gaskin, Felicia (2013) Autoimmunity, end organ damage, and the origin of autoantibodies and autoreactive T cells in systemic lupus erythematosus. Discov Med 15:85-92
Ge, Yan; Brown, Michael G; Wang, Hongyang et al. (2012) Genetic approach to study lupus glomerulonephritis. Methods Mol Biol 900:271-90
Fu, Shu Man; Deshmukh, Umesh S; Gaskin, Felicia (2011) Pathogenesis of systemic lupus erythematosus revisited 2011: end organ resistance to damage, autoantibody initiation and diversification, and HLA-DR. J Autoimmun 37:104-12
Deshmukh, Umesh S; Sim, Davis L; Dai, Chao et al. (2011) HLA-DR3 restricted T cell epitope mimicry in induction of autoimmune response to lupus-associated antigen SmD. J Autoimmun 37:254-62
Jiang, Chao; Deshmukh, Umesh S; Gaskin, Felicia et al. (2010) Differential responses to Smith D autoantigen by mice with HLA-DR and HLA-DQ transgenes: dominant responses by HLA-DR3 transgenic mice with diversification of autoantibodies to small nuclear ribonucleoprotein, double-stranded DNA, and nuclear antigens. J Immunol 184:1085-91
Sharma, Rahul; Sung, Sun-sang Joe; Fu, Shu Man et al. (2009) Regulation of multi-organ inflammation in the regulatory T cell-deficient scurfy mice. J Biomed Sci 16:20
Bagavant, Harini; Fu, Shu Man (2009) Pathogenesis of kidney disease in systemic lupus erythematosus. Curr Opin Rheumatol 21:489-94

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