Abstract

Pilot Project 2--The primary aim of this study is to develop novel molecular imaging methodology to visualize and quantify the expression and activity of tyrosine kinase growth factor receptor and mitochondria uncoupling proteins in metastasis. The Met tyrosine kinase growth factor receptor, its ligand hepatocyte and Mimp (ref), a novel Met-HGF/SF-induced protein will serve as a model. Using novel molecular imaging modalities we will analyze the effects of Met and HGF/SF overexpression on mammary tumors and metastasis formation. Subsequently, using a Tet inducible Mimp we will examine the effects of Mimp expression on Met-HGF/SF-induced metastasis. Preliminary results suggest that Mimp reduces Met-HGF/SF-induced metastasis. The combined expression of Met and Mimp which is inhibitory and its down-stream effects may shed light on the involvement of mitochondrial uncoupling activities in regulating HGF/SF-induced metastasis.
Our specific aims are: 1) to image overexpression of Met and HGF/SF in an in vitro invasion process and in tumor and metastasis formation. 2) to characterize the effect of Mimp induction on the tumorigenic/metastatic potential in in-vitro and in vivo systems. 3) to develop molecular imaging modalities for Mimp-luciferase and Mimp-DsRed in tumorigenicity and metastasis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center (P50)
Project #
1P50CA093990-01
Application #
6611777
Study Section
Special Emphasis Panel (ZCA1)
Project Start
2002-06-14
Project End
2007-03-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Keith, Lauren; Ross, Brian D; Galbán, Craig J et al. (2016) Semiautomated Workflow for Clinically Streamlined Glioma Parametric Response Mapping. Tomography 2:267-275
Nyati, Shyam; Chator, Areeb; Schinske, Katerina et al. (2016) A Requirement for ZAK Kinase Activity in Canonical TGF-? Signaling. Transl Oncol 9:473-481
Joshi, Bishnu P; Pant, Asha; Duan, Xiyu et al. (2016) Multimodal Video Colonoscope for Targeted Wide-Field Detection of Nonpolypoid Colorectal Neoplasia. Gastroenterology 150:1084-1086
Al-Dujaili, Saja A; Koh, Amy J; Dang, Ming et al. (2016) Calcium Sensing Receptor Function Supports Osteoblast Survival and Acts as a Co-Factor in PTH Anabolic Actions in Bone. J Cell Biochem 117:1556-67
Stacer, A C; Fenner, J; Cavnar, S P et al. (2016) Endothelial CXCR7 regulates breast cancer metastasis. Oncogene 35:1716-24
Luker, K E; Pata, P; Shemiakina, I I et al. (2015) Comparative study reveals better far-red fluorescent protein for whole body imaging. Sci Rep 5:10332
Chang, S Laura; Cavnar, Stephen P; Takayama, Shuichi et al. (2015) Cell, isoform, and environment factors shape gradients and modulate chemotaxis. PLoS One 10:e0123450
Stacer, Amanda C; Wang, Hanxiao; Fenner, Joseph et al. (2015) Imaging Reporters for Proteasome Activity Identify Tumor- and Metastasis-Initiating Cells. Mol Imaging 14:414-28
Van Dort, Marcian E; Galbán, Stefanie; Wang, Hanxiao et al. (2015) Dual inhibition of allosteric mitogen-activated protein kinase (MEK) and phosphatidylinositol 3-kinase (PI3K) oncogenic targets with a bifunctional inhibitor. Bioorg Med Chem 23:1386-94
Wang, Lizhong; Liu, Runhua; Ye, Peiying et al. (2015) Intracellular CD24 disrupts the ARF-NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nat Commun 6:5909

Showing the most recent 10 out of 161 publications