The specific aims of this proposal are 1)To collect, process, bank and distribute human colorectal neoplasmsand matched normal tissue samples to investigators in the Vanderbilt Gl SPORE and other Gl SPORES;2)To collect and bank portions of polyps and biopsies of grossly normal colorectal mucosa to facilitateresearch in adenoma recurrence; 3)To perform quality control to ensure that the relevant tissue is supplied tothe researcher and that tissues are suitable for the planned research (not necrotic or involved byunsuspected disease processes); 4)To protect patient confidentiality through use of an explicit consent formthat specifically addresses use of extraneous tissue for research purposes and through de-identification ofspecimens; 5)To work with the Biostatistics/Bioinformatics Core to establish an informatics strategy fornetworking of requests, specimen tracking, extraction of de-identified data relating to specimens of interestand linkage to research data. 6) To provide laser capture microdissection services to the Gl SPOREinvestigators; 7)To provide tissue microarray services to the Gl SPORE investigators and provide TMA slidesto investigators at other institutions (such as the H. Lee Moffitt Cancer Center) and other Gl SPOREs; 8)Toprovide expertise in evaluation of histopathology of mouse models of colorectal neoplasia and correlationwith human disease; 10)To provide expertise in developing, performing and evaluating immunohistochemicalstains for Gl SPORE investigators. The Gl SPORE Tissue Core at VUMC has partnered with othermechanisms for tissue collection at VUMC under Dr. Washington's direction: the Human Tissue Acquisitionand Pathology Shared Resource of the Vanderbilt-lngram Comprehensive Cancer Center, the TissueMorphology SubCore for the Digestive Disease Research Center and the VUMC-led Western Division of theCooperative Human Tissue Network. Mechanisms and standard operating procedures for collection ofhuman Gl tissue, quality assurance and immunohistochemistry have been used to support all five SPOREprojects in the previous funding period. By partnering with other tissue and histology resources at VUMC, theGl SPORE Tissue Core is able to provide high quality services to SPORE investigators in a highly costeffective manner.
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