Abstract

The function of the Molecular Biology Core is to provide the Center for Study of Opioid Receptors and Drugs of Abuse (CSORDA) with resources related to the use of recombinant DNA and adjunct methodologies. In broad terms, these resources will be used by members of the various Scientific Components for experiments designed to analyze and characterize genes encoding members of the opioid receptor family and their peptide ligands. The Molecular Biology Core Director and staff will support CSORDA Components by designing and implementing site-directed mutagenesis protocols, genetically engineer chimeric and epitope-tagged proteins, construct eucaryotic viral and plasmid-based expression constructs, and genotype transgenic and mutant mice strains. In addition, the Molecular Biology Core will consult with the Principle Investigators and Core supervisors on overall strategies for individual projects and provide training and support in specific molecular techniques to students, technicians, visiting scientists, and collaborators working outside the Center.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Specialized Center (P50)
Project #
2P50DA005010-16
Application #
6555697
Study Section
Special Emphasis Panel (ZDA1)
Project Start
2002-08-01
Project End
2007-07-31
Budget Start
Budget End
Support Year
16
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Severino, Amie; Chen, Wenling; Hakimian, Joshua K et al. (2018) Mu-opioid receptors in nociceptive afferents produce a sustained suppression of hyperalgesia in chronic pain. Pain 159:1607-1620
Ben Hamida, Sami; Mendonça-Netto, Sueli; Arefin, Tanzil Mahmud et al. (2018) Increased Alcohol Seeking in Mice Lacking Gpr88 Involves Dysfunctional Mesocorticolimbic Networks. Biol Psychiatry 84:202-212
Lee, Kevin; Vuong, Helen E; Nusbaum, David J et al. (2018) The gut microbiota mediates reward and sensory responses associated with regimen-selective morphine dependence. Neuropsychopharmacology 43:2606-2614
Maroteaux, G; Arefin, T M; Harsan, L-A et al. (2018) Lack of anticipatory behavior in Gpr88 knockout mice showed by automatized home cage phenotyping. Genes Brain Behav 17:e12473
Ehrlich, Aliza T; Semache, Meriem; Bailly, Julie et al. (2018) Mapping GPR88-Venus illuminates a novel role for GPR88 in sensory processing. Brain Struct Funct 223:1275-1296
Becker, Jérôme A J; Kieffer, Brigitte L; Le Merrer, Julie (2017) Differential behavioral and molecular alterations upon protracted abstinence from cocaine versus morphine, nicotine, THC and alcohol. Addict Biol 22:1205-1217
Bakhurin, Konstantin I; Goudar, Vishwa; Shobe, Justin L et al. (2017) Differential Encoding of Time by Prefrontal and Striatal Network Dynamics. J Neurosci 37:854-870
Lalanne, L; Ayranci, G; Filliol, D et al. (2017) Kappa opioid receptor antagonism and chronic antidepressant treatment have beneficial activities on social interactions and grooming deficits during heroin abstinence. Addict Biol 22:1010-1021
Dagnew, Robel; Lin, Yin-Ying; Agatep, Jerikko et al. (2017) CerebraLux: a low-cost, open-source, wireless probe for optogenetic stimulation. Neurophotonics 4:045001
Boulos, Laura-Joy; Darcq, Emmanuel; Kieffer, Brigitte Lina (2017) Translating the Habenula-From Rodents to Humans. Biol Psychiatry 81:296-305

Showing the most recent 10 out of 117 publications