The host immunologic response to oral microorganisms may play a significant role in the pathogenesis of periodontal diseases. It is not clear whether this response is primarily protective or at times may have destructive components. In the present investigation, the humoral and cellular immunologic response to the predominant microorganisms isolated from sites of active destruction will be determined. Samples of saliva, blood, and gingival tissue from the diseased site(s) will be collected prior to and after therapy. Individual microbial species present in destructive and nondestructive sites will be compared for their relative ability to stimulate local and/or systemic cellular/humoral immune response. Reactions to a standard battery of suspected periodontopathic microorganisms will also be determined to provide some cross-sectional information between various patients in the studies. However, the priority analyses will be to antigens of the host's indigenous microbiota. Computer-assisted analyses of changes in immunologic responses will be compared to changes in microbial composition and clinical status of disease. Also, the effect of both mechanical and/or antibiotic therapy on these responses will be determined longitudinally for the individual patients. Any consistent patterns in immunologic responses to the microbial antigens may be useful in determining etiologic agent(s) of specific periodontal diseases. It is conceivable that imunologic responses may reflect altered host-microbe interactions that precipitate the onset of disease. Thus, the use of changes in antibody levels or in cellular immune responses to suspected pathogens as an in vitro laboratory diagnostic test can be evaluated.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE004881-09
Application #
4692782
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Forsyth Institute
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
Craig, Ronald G; Yip, Julie K; Mijares, Dindo Q et al. (2003) Progression of destructive periodontal diseases in three urban minority populations: role of clinical and demographic factors. J Clin Periodontol 30:1075-83
Craig, Ronald G; Boylan, Robert; Yip, Julie et al. (2002) Serum IgG antibody response to periodontal pathogens in minority populations: relationship to periodontal disease status and progression. J Periodontal Res 37:132-46
Feres, M; Haffajee, A D; Allard, K et al. (2001) Change in subgingival microbial profiles in adult periodontitis subjects receiving either systemically-administered amoxicillin or metronidazole. J Clin Periodontol 28:597-609
Craig, R G; Boylan, R; Yip, J et al. (2001) Prevalence and risk indicators for destructive periodontal diseases in 3 urban American minority populations. J Clin Periodontol 28:524-35
Cugini, M A; Haffajee, A D; Smith, C et al. (2000) The effect of scaling and root planing on the clinical and microbiological parameters of periodontal diseases: 12-month results. J Clin Periodontol 27:30-6
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Ximenez-Fyvie, L A; Haffajee, A D; Socransky, S S (2000) Comparison of the microbiota of supra- and subgingival plaque in health and periodontitis. J Clin Periodontol 27:648-57
Sakellari, D; Goodson, J M; Kolokotronis, A et al. (2000) Concentration of 3 tetracyclines in plasma, gingival crevice fluid and saliva. J Clin Periodontol 27:53-60
Ximenez-Fyvie, L A; Haffajee, A D; Som, S et al. (2000) The effect of repeated professional supragingival plaque removal on the composition of the supra- and subgingival microbiota. J Clin Periodontol 27:637-47
Socransky, S S; Haffajee, A D; Smith, C et al. (2000) Microbiological parameters associated with IL-1 gene polymorphisms in periodontitis patients. J Clin Periodontol 27:810-8

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