One of the principal factors implicated in the cariogenicity of Streptococcus mutans is the enzyme; glucosyltransferase. The ultimate goal of this project is to gain a more thorough understanding of the role of the Streptococcus mutans GS-5 derived glucosyltransferase in adherence, cell aggregation, and cariogenesis. Using recombinant DNA technology to clone the gene coding for this enzyme, which synthesizes water insoluble glucan, it will be possible to study the regulation of expression on the nucleotide sequence level. More importantly, it will be possible, using site-directed, in vitro mutagenesis with subsequent transfer of the mutation to S. mutants GS-5, to create a series of well-characterized glucosyltransferase mutants in isogenic backgrounds. Using these mutants in both in vitro and in vivo studies it will now be possible to assess the role of both glucosyltransferase and insoluble glucan in adherence, aggregation, and cariogenesis without the concern of other factors influencing the results. From these studies, an understanding of the pathogenesis of dental caries will be realized and information will be gained that can aid in the prevention and eventual elimination of caries.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE007003-05
Application #
3917217
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Rochester
Department
Type
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627
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O'Connell, A C; Bowen, W H (1994) Composition and flow rate of saliva and caries development in young rats following administration of lithium. Caries Res 28:342-7

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