Abstract

Samples of plaque will be taken from carious and noncarious exposed root surfaces and the total viable count will be assayed on blood agar plates grown anaerobically after lightly ultrasonicating the specimen. Stimulated whole and paratod saliva samples will be taken over ice for a fixed time period and their microbiology assayed. The bacteria to be identified include: Streptococci, actinomyces, lactobacilli and veillonella. For Streptococci (S. mutans, milleri, mitior, and sanguis) samples will be cultured on TYC medium. The different morphological types will be counted. Colonies representative of each of the main morphological types will be gram stained and others picked off, grown in nutrient broth (Brain Heart infusion or Todd Hewitt) and identified in the API 20 streptococci identification strip. Not all streptococci will fall into the major known species and these will be noted. Counts of each of the identified species will be expressed as a percentage of the total viable count (TVC) detected on the blood agar plates. For actinomyces cultures will be grown on blood agar and representative colonies analyzed against specific anti-sera (kindly donated by Dr. G. Bowden) which will distinguish by agglutination between A. Israelii, A. Naeslundii (1 and 2), A. Viscosus and A. Odontolyticus. Lactobacilli, samples will be plated out on Rogosas medium. The total count on Rogosas medium can be expressed in relation to the total viable count on blood agar to give the percentage of lactobacilli. Speciation (C. casei, L. acidophilus and others) will be performed using a limited number of biochemical tests (details to be supplied by Dr. G. Bowden). Veillonella will be isolated on blood agar containing 7.5 micrograms per ml of Vancomycin. Representative colonies will be gram stained to identify those which are gram negative cocci, and the count expressed as for streptococci. Representative colonies will be picked off, grown up in nutrient broth and stored for more detailed analysis at a later date.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE007010-03
Application #
3963913
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Levy, S M; Maurice, T J; Jakobsen, J R (1993) Feeding patterns, water sources and fluoride exposures of infants and 1-year-olds. J Am Dent Assoc 124:65-9
Levy, S M; Jensen, M E (1990) A clinical evaluation of the restoration of root surface caries. Spec Care Dentist 10:156-60
Ekstrand, J; Spak, C J (1990) Fluoride pharmacokinetics: its implications in the fluoride treatment of osteoporosis. J Bone Miner Res 5 Suppl 1:S53-61
Spak, C J; Sjostedt, S; Eleborg, L et al. (1990) Studies of human gastric mucosa after application of 0.42% fluoride gel. J Dent Res 69:426-9
Almqvist, H; Wefel, J S; Lagerlof, F (1990) Root hard-tissue demineralization rate measured by 125I absorptiometry: comparison with lesion-depth measurements. J Dent Res 69:1519-21
Bowden, G H; Ekstrand, J; McNaughton, B et al. (1990) Association of selected bacteria with the lesions of root surface caries. Oral Microbiol Immunol 5:346-51
Ekstrand, J; Spak, C J; Vogel, G (1990) Pharmacokinetics of fluoride in man and its clinical relevance. J Dent Res 69 Spec No:550-5;discussion 556-7
Oliveby, A; Twetman, S; Ekstrand, J (1990) Diurnal fluoride concentration in whole saliva in children living in a high- and a low-fluoride area. Caries Res 24:44-7
Oliveby, A; Lagerlof, F; Ekstrand, J et al. (1989) Studies on fluoride excretion in human whole saliva and its relation to flow rate and plasma fluoride levels. Caries Res 23:243-6
Ekstrand, J (1989) Fluoride intake in early infancy. J Nutr 119:1856-60

Showing the most recent 10 out of 23 publications