Abstract

This core will be responsible for producing recombinant members of the TGF-beta family and at least one functional domain of the fibronectin molecule for Center members. This will involve transfection and selection of producer cell lines, and production and purification of recombinant proteins. In addition, the core will provide advice on the molecular biology aspects of investigator projects. The core will consist of one full time experienced SRA II, and a 50% SRA II under the supervision of Dr. Derynck. All projects except Project 1 will utilize this core. It will be essential to Projects 2-5 and to Pilot Project 6.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE010306-04
Application #
5210204
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Kamiya, Nobuhiro; Jikko, Akitoshi; Kimata, Koji et al. (2002) Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. J Bone Miner Res 17:1832-42
Choy, L; Skillington, J; Derynck, R (2000) Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. J Cell Biol 149:667-82
Bullard, K M; Lund, L; Mudgett, J S et al. (1999) Impaired wound contraction in stromelysin-1-deficient mice. Ann Surg 230:260-5
Filvaroff, E; Erlebacher, A; Ye, J et al. (1999) Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126:4267-79
Miettinen, P J; Chin, J R; Shum, L et al. (1999) Epidermal growth factor receptor function is necessary for normal craniofacial development and palate closure. Nat Genet 22:69-73
Jikko, A; Harris, S E; Chen, D et al. (1999) Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 14:1075-83
Iwasaki, M; Jikko, A; Le, A X (1999) Age-dependent effects of hedgehog protein on chondrocytes. J Bone Joint Surg Br 81:1076-82
Ratcliff, P A; Johnson, P W (1999) The relationship between oral malodor, gingivitis, and periodontitis. A review. J Periodontol 70:485-9
Werb, Z; Vu, T H; Rinkenberger, J L et al. (1999) Matrix-degrading proteases and angiogenesis during development and tumor formation. APMIS 107:11-8
Suen, P W; Ilic, D; Caveggion, E et al. (1999) Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages. J Cell Sci 112 ( Pt 22):4067-78

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