Abstract

This core will be responsible for producing recombinant members of the TGF-beta family and at least one functional domain of the fibronectin molecule for Center members. This will involve transfection and selection of producer cell lines, and production and purification of recombinant proteins. In addition, the core will provide advice on the molecular biology aspects of investigator projects. The core will consist of one full time experienced SRA II, and a 50% SRA II under the supervision of Dr. Derynck. All projects except Project 1 will utilize this core. It will be essential to Projects 2-5 and to Pilot Project 6.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Specialized Center (P50)
Project #
5P50DE010306-05
Application #
6238506
Study Section
Project Start
1996-12-01
Project End
1999-10-31
Budget Start
Budget End
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Kamiya, Nobuhiro; Jikko, Akitoshi; Kimata, Koji et al. (2002) Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. J Bone Miner Res 17:1832-42
Choy, L; Skillington, J; Derynck, R (2000) Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. J Cell Biol 149:667-82
Suen, P W; Ilic, D; Caveggion, E et al. (1999) Impaired integrin-mediated signal transduction, altered cytoskeletal structure and reduced motility in Hck/Fgr deficient macrophages. J Cell Sci 112 ( Pt 22):4067-78
Johnson, P W; Lancero, H (1999) Function of gingival fibroblasts and periodontal ligament cells in the presence of methyl mercaptan. Quintessence Int 30:343-9
Gerber, H P; Vu, T H; Ryan, A M et al. (1999) VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat Med 5:623-8
Bullard, K M; Lund, L; Mudgett, J S et al. (1999) Impaired wound contraction in stromelysin-1-deficient mice. Ann Surg 230:260-5
Filvaroff, E; Erlebacher, A; Ye, J et al. (1999) Inhibition of TGF-beta receptor signaling in osteoblasts leads to decreased bone remodeling and increased trabecular bone mass. Development 126:4267-79
Miettinen, P J; Chin, J R; Shum, L et al. (1999) Epidermal growth factor receptor function is necessary for normal craniofacial development and palate closure. Nat Genet 22:69-73
Jikko, A; Harris, S E; Chen, D et al. (1999) Collagen integrin receptors regulate early osteoblast differentiation induced by BMP-2. J Bone Miner Res 14:1075-83
Iwasaki, M; Jikko, A; Le, A X (1999) Age-dependent effects of hedgehog protein on chondrocytes. J Bone Joint Surg Br 81:1076-82

Showing the most recent 10 out of 39 publications