Abstract

The overall aim of the Cell Culture and Molecular Probes Core Laboratory is to provide human tissues, prepare primary cultures, and maintain stocks of cultured cells for distribution to all participants in the Center, to prepare polyclonal antibodies and to provide expertise in instrumentation for microscopic analysis of normal and CF cells and tissues.
The specific aims of Core B are as follows: (1) to procure human tissues from normal and CF patients for use by all participants in the Center, (2) to provide cell lines including T84, TBE-1, and CFPAC as well as primary cultures of various experiments, (3) to inject rabbits with antigens provided by investigators in these projects and to bleed the animals and provide serum. (4) to provide standard microscopic analysis including brightfield, phase contrast and Nomarski, immunofluorescence image analysis, scanning confocal microscopy, and electron microscopy. Therefore, Core B will be a major resource for the Center and will constitute an integrated and essential component of each individual project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Specialized Center (P50)
Project #
5P50DK042017-05
Application #
3776634
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Type
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Schwiebert, E M; Benos, D J; Fuller, C M (1998) Cystic fibrosis: a multiple exocrinopathy caused by dysfunctions in a multifunctional transport protein. Am J Med 104:576-90
Ji, H L; DuVall, M D; Patton, H K et al. (1998) Functional expression of a truncated Ca(2+)-activated Cl- channel and activation by phorbol ester. Am J Physiol 274:C455-64
Tousson, A; Fuller, C M; Benos, D J (1996) Apical recruitment of CFTR in T-84 cells is dependent on cAMP and microtubules but not Ca2+ or microfilaments. J Cell Sci 109 ( Pt 6):1325-34
Jovov, B; Ismailov, I I; Benos, D J (1995) Cystic fibrosis transmembrane conductance regulator is required for protein kinase A activation of an outwardly rectified anion channel purified from bovine tracheal epithelia. J Biol Chem 270:1521-8
Cunningham, S A; Awayda, M S; Bubien, J K et al. (1995) Cloning of an epithelial chloride channel from bovine trachea. J Biol Chem 270:31016-26
Sanchez-Olea, R; Fuller, C; Benos, D et al. (1995) Volume-associated osmolyte fluxes in cell lines with or without the anion exchanger. Am J Physiol 269:C1280-6
Singh, A K; Venglarik, C J; Bridges, R J (1995) Development of chloride channel modulators. Kidney Int 48:985-93
Venglarik, C J; Schultz, B D; Frizzell, R A et al. (1994) ATP alters current fluctuations of cystic fibrosis transmembrane conductance regulator: evidence for a three-state activation mechanism. J Gen Physiol 104:123-46
Bradbury, N A; Cohn, J A; Venglarik, C J et al. (1994) Biochemical and biophysical identification of cystic fibrosis transmembrane conductance regulator chloride channels as components of endocytic clathrin-coated vesicles. J Biol Chem 269:8296-302
Reyes, J G; Santander, M; Martinez, P L et al. (1994) A fluorescence method to determine picomole amounts of Zn(II) in biological systems. Biol Res 27:49-56

Showing the most recent 10 out of 32 publications